Method for quickly identifying resistance of single pepper plant to gellan anthracnose by using isolated leaves

An isolated leaf and anthracnose technology is applied in the field of rapid identification of individual pepper plants' resistance to Glycystis anthracnose and Capsicum anthracnose. good, fast effect

Inactive Publication Date: 2019-04-16
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the deficiencies in the prior art, the object of the present invention is to provide a method for quickly identifying the resistance of individual peppers to G. The technical problem of low efficiency and large limitations of the identification method of resistance

Method used

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  • Method for quickly identifying resistance of single pepper plant to gellan anthracnose by using isolated leaves

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Embodiment 1

[0054] The present embodiment provides a method for quickly identifying the resistance of capsicum individual plants to glyospora anthracnose by using detached leaves. The method specifically includes the following steps:

[0055] Step 1, selecting capsicum disease-resistant strain R25 as the disease-resistant control, capsicum-susceptible strain R24 as the susceptible control, selecting the capsicum seeds of the disease-resistant control and the capsicum seeds of the susceptible control;

[0056] In step 2, the pepper samples CM334 and R9 seeds with unknown resistance to G. anthracnose, the pepper seeds of the disease-resistant control and the pepper seeds of the susceptible control are cultivated and planted respectively;

[0057] The specific cultivation and planting process is as follows: Soak pepper seeds in warm soup at 55°C for 20 minutes, soak in clear water for 5 hours, germinate in an artificial climate box at 28°C in total darkness, and sow the seeds in plug trays af...

Embodiment 2

[0083] This embodiment provides a method for quickly identifying the resistance of a single pepper plant to G. anthracnose by using detached leaves. The specific steps of the method are basically the same as in Example 1. The only difference is that in step 2, the resistance to G. anthracnose Pepper samples with unknown disease resistance were R30 and AA3 seeds.

[0084] According to the method provided in this embodiment, the detection results are as follows:

[0085] The disease index of R25 is 5, and it is a disease-resistant strain.

[0086] The disease index of R24=60, which is a susceptible strain.

[0087] The disease index of R30=5, it is a disease-resistant strain.

[0088] The disease index of AA3 is 32, which means it is a disease-resistant strain.

[0089] The above results are compared with the results of acupuncture identification (the disease index of R30=0, which is a disease-resistant strain; the disease index of AA3=24, which is a disease-resistant strain)...

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Abstract

The invention provides a method for quickly identifying the resistance of a single pepper plant to gellan anthracnose by using isolated leaves, which comprises the following steps of: using isolated functional leaves with stems in a pepper seedling period as a sample to be detected; taking pathogenic bacteria of the coleobacteria anthracnose and inoculating the pathogenic bacteria to the back surfaces of the leaves of the sample to be detected; then placing the sample to be detected in an artificial climate box at a temperature of 28+ / -2 DEG C, carrying out dark culture for 48 hours to obtaina lesion sample, and measuring a lesion diameter of the lesion sample; wherein the sample to be detected is from a pepper plant at a 12-13 leaf seedling stage. The method has the advantages of simpleand rapid steps, stability, reliability and good repeatability, is particularly suitable for molecular marker development, disease resistance identification and genetic analysis of single pepper breeding plant (or strain) such as transgenic strains and the like, can well improve the disease resistance identification and screening efficiency of the pepper, and accelerates the breeding process.

Description

technical field [0001] The invention belongs to the field of vegetable breeding, and relates to capsicum anthracnose, in particular to a method for quickly identifying the resistance of a capsicum individual plant to the capsicum anthracnose by using isolated leaves. Background technique [0002] Capsicum annuum L. is caused by C. gloeosporiodes, C. acutatum, C. anthracnose (C. capsici) and spherical anthracnose (C.coccodes) and other global fungal diseases caused by several species, which commonly occur, and the most serious infection is the pathogen of glyospora anthracnose, which has a wide host range and is the main cause of harm to peppers and sweet peppers. one of the diseases. Under the climatic conditions of high temperature and high humidity, especially in the main pepper producing areas such as the tropics and subtropics, the disease is more common, the damage time is long, and the economic loss is large. In China, India, Indonesia, South Korea and other regions,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
CPCC12Q1/04C12Q1/18G01N2333/37
Inventor 巩振辉孙建田李大伟罗德旭李洋
Owner NORTHWEST A & F UNIV
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