Detection method of resonance energy transfer fluorescence

Abstract

The invention discloses a detection method of resonance energy transfer fluorescence. The method comprises the following steps in order: S1, a light source emits a beam to pass through a first opticalfilter, the light of most light source wavelength is transmitted, the beam passes through a transflective dichroic filter a to reflect the exciting light in 365nm, a beam direction is changed, and the fluorescence substances irradiated to a detection line and a quality control line of a to-be-detected sample are focused under the effect of a convex lens; S2, the detected to-be-detected sample produces the fluorescence in 690nm by performing excitation energy resonance transfer on the quantum dot donor under the irradiation of the exciting light. Through the detection method disclosed by the invention, the light intensity information in different characters can be collected by adopting the resonance energy transfer fluorescence detection method, the interference is reduced by processing the light intensity signals with different wavelengths, and the detection sensitivity is greatly increased; the light path design does not limit the wavelength of the optical device, the device parameters can be replaced according to the optical characteristics of donor and receptor group with different resonance energy transfer fluorescence, and the detection method has universality.

Description

technical field [0001] The invention relates to the field of optical detection channel design, in particular to a resonance energy transfer fluorescence detection method. Background technique [0002] Fluorescence Resonance Energy Transfer (FRET, Fluorescence Resonance Energy Transfer) refers to a non-radiative energy transition, which transfers energy from an excited fluorophore to another non-radiatively through the electric-dipole interaction between molecules. Physical phenomena of fluorophores. FRET has the characteristics of high resolution, high sensitivity, good reproducibility and strong specificity. It is mainly used in the research of the structure, function and properties of biological macromolecules, as well as the determination of inorganic ions, nucleic acid detection, immunoassay, quantitative analysis and other fields. [0003] However, the existing detection method of resonance energy transfer fluorescence has the following defects: the intensity of reson...

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Problems solved by technology

[0003] However, the existing detection method of resonance energy transfer fluorescence has the following defects: the intensity of resonance energy transfer fluorescence is weak, the excitation efficiency is not high, and there are many interference problems; in view of these defects, we design a detection method of resonance energy transfer fluorescence as very necessary

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