Culture medium for 3D culture of ovarian cancer tissue

Abstract

The invention discloses a culture medium for 3D culture of ovarian cancer tissue, the tissue is prepared from cytokine B27, N-acetylcysteine, R-spondin 1, A83-01, epidermal growth factors, glutamine,N2Supplement, estrogen, progesterone and vitamin E. The culture medium includes various cytokines and signal pathway regulatory factors which are needed by ovarian cancer tissue cell culture, mutually, directly and closely affect each other and coordinate with each other, so that the ovarian cancer tissue cells can better display the inherent active characteristics in the process of culture, and the overall characteristic highly similar to the living ovarian cancer tissue is achieved, and ovarian tumor cells subjected to 3D culture with the culture medium are clustered and are hypoxic in the middle and similar to the ovarian tumor tissue.

Description

technical field [0001] The invention belongs to the field of biomedicine and discloses a medium for 3D culture of ovarian cancer tissue. Background technique [0002] Currently, the in vitro methods for screening and evaluating antineoplastic drugs are mainly 2D culture systems of tumor cells, while the methods for in vivo screening and evaluation mainly use animal models. In 2D culture, tumor cells are very different from their in vivo state in terms of morphology, differentiation, expansion, metabolic response to stimuli, and gene protein expression. For the animal evaluation system, the success rate of inoculation is low, and the experimental cycle Long, but also due to the large species differences with humans, it cannot fully reflect the metabolic process, efficacy and side effects of drugs in the human body. [0003] As a new method for recent oncology research, human tissue organoid culture technology uses mature human cells to cultivate human tissue organoids under ...

AI Technical Summary

Problems solved by technology

In 2D culture, tumor cells are very different from their in vivo state in terms of morphology, differentiation, expansion, metabolic response to stimuli, and gene protein expression. For the animal evaluation system, the success rate of inoculation is low, and the experimental cycle Long, but also due to the large species differences with humans, it cannot fully reflect the metabolic process, efficacy and side effects of drugs in the human body.

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