Method of enhancing immunosuppression by over-expressing IDO by cs and application thereof

A technology of stem cells and immune enhancement, applied in the field of stem cell preparation, can solve the problems of unsatisfactory clinical immunotherapy effect, etc., and achieve the effect of enhancing immunosuppressive ability, reducing the number of clinical use, and improving immunosuppressive ability.

Inactive Publication Date: 2019-05-03
杭州荣泽生物科技集团有限公司
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  • Abstract
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Problems solved by technology

The reason is that the effect of clinical immunotherapy is still not satisfactory

Method used

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  • Method of enhancing immunosuppression by over-expressing IDO by cs and application thereof
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  • Method of enhancing immunosuppression by over-expressing IDO by cs and application thereof

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Embodiment Construction

[0042] The present invention will be further described below in conjunction with specific embodiments, it should be understood that they are only examples, and the protection scope of the present invention is not limited thereto. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, equivalent changes and modifications made without departing from the spirit and scope of the present invention shall fall within the scope of the present invention.

[0043] The methods described in the following examples are conventional methods unless otherwise specified. The reagent consumables required in the examples can be purchased in the market unless otherwise specified. The various ways of obtaining biological materials in the examples are only experimental ways to achieve the purpose of specific disclosure, and should not limit the source of biological materials in the present ...

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Abstract

In order to solve the problem of limited stem cell immunosuppressive ability existing in the prior art, the present invention relates to the fields of adult stem cell and cell therapy, and relates toa novel application of mesenchymal stem cells, in particular to a method for enhancing immunosuppression by over-expressing IDO by umbilical cord mesenchymal stem cells. The method is characterized byconstructing a lentivirus overexpressing an IDO gene, constructing the umbilical cord mesenchymal stem cell overexpressing IDO by a lentiviral transfection system, and adding a gene opening agent; and co-culturing the umbilical cord mesenchymal stem cell expressing the IDO and the immune cells, which greatly improves the immunosuppressive ability of the umbilical cord mesenchymal stem cells, reduces the number of clinical use of stem cells, and save a lot of time, labor and material costs.

Description

technical field [0001] The invention relates to the field of stem cell preparation, in particular, the invention relates to a method for overexpressing IDO in umbilical cord mesenchymal stem cells to enhance immune suppression. Background technique [0002] In modern times, organ transplantation has become an effective means of treating organ failure, but immune rejection has always been one of the biggest problems affecting the field of organ transplantation. Although haploidentical bone marrow transplantation and removal of αβTCR cells in hematopoietic stem cells have made some progress in the treatment of graft-versus-host disease (GVHD), the therapeutic effect still needs further clinical research. Mesenchymal stem cells (MSCs) have the function of immune regulation. Through the direct interaction between cells and the indirect effect of secreting cytokines, they can inhibit the proliferation of T cells and immune response, thereby exerting the function of immune reconst...

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Application Information

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IPC IPC(8): C12N15/867C12N15/53C12N5/10C12N5/0775A61P37/06
Inventor 陈相波雷鸣田朋飞
Owner 杭州荣泽生物科技集团有限公司
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