A kind of separation and purification of Rhododendron erinaceus mycorrhizal fungus strain dps-a and its inoculation application method

A technology of DPS-A and mycorrhizal fungi, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., to achieve the effects of short strain isolation and purification cycle, simple technical operation, and improved seedling supply ability
CN109722390BActive Publication Date: 2022-04-29浙江省大盘山国家级自然保护区管理局
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
浙江省大盘山国家级自然保护区管理局
Publication Date
2022-04-29

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Abstract

The invention discloses the separation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS‑A and its inoculation application method, comprising the following steps: (1) selecting mycorrhiza and weighing; (2) sterilizing the surface of the root system; (3) (4) root tissue homogenate; (5) strain purification; (6) preliminary screening of strains; (7) preparation of bacterial agents; (8) strain inoculation; (9) detection of inoculation effects. The advantages are: the strain isolation and purification period is short, the technical operation is simple, and the high efficiency and large-scale operation of the isolation and purification of Rhododendron erinaceus mycorrhizal fungi are realized.
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Description

technical field

[0001] The invention relates to the separation and purification of Rhododendron erinaceus mycorrhizal fungus strain DPS-A and its application method for inoculation. Background technique

[0002] Mycorrhiza is a symbiosis formed by the vegetative roots of higher plants and mycorrhizal fungi, and is a very common natural phenomenon. Mycorrhizae can expand the nutrient absorption surface area of ​​the host plant root system, promote the production of various growth stimulating substances such as auxin, enhance the resistance of plant roots to diseases and insect pests, and improve the stress resistance of plants to harmful substances such as heavy metals, etc., thereby promoting plant growth. Individual growth and increase its resilience. The root system of most Rhododendron (Ericaceae) plants does not have a root hair structure, but consists of a large number of slender fibrous hairy roots with a limited surface area for nutrient absorption, and thus has a hi...

Claims

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