A detection kit for non-small cell lung cancer and its application

A non-small cell lung cancer and detection reagent technology, which is applied in the direction of measuring devices, microbial determination/inspection, instruments, etc., can solve the problems of complex detection mechanism, false positive, high sample requirements, etc., to overcome cumbersome operation and high specificity , good sensitivity

Active Publication Date: 2022-04-26
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its use is limited due to high false alarm rate, potential overdiagnosis, high cost, and possible radiation exposure hazards.
And tissue biopsy, such as based on lung cancer markers, such as SALL4 (patent number CN105044348A), EIF4G1 (CN105624275A) and other detections, has positive significance for the treatment of lung cancer, but it is traumatic to patients and cannot be used for early diagnosis of lung cancer.
Most of the emerging in vitro detection of lung cancer is based on the content of lung cancer markers micRNA in serum, such as miR-106a, miR-20a, (CN106350582A) miR 3613 (CN105603117A), etc., to achieve early diagnosis of lung cancer and screening of high-risk groups, but the use of nucleic acid The detection mechanism of the detection is relatively complex, the sample requirements are high and false positives are prone to occur
In summary, the current lung cancer detection methods have major defects, and there is no effective clinical method for early diagnosis of lung cancer and screening of high-risk groups

Method used

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  • A detection kit for non-small cell lung cancer and its application
  • A detection kit for non-small cell lung cancer and its application
  • A detection kit for non-small cell lung cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0148] 1. Serum sample collection:

[0149] Collect clinical non-small cell lung cancer patient serum and normal human serum:

[0150] The serum of 57 patients with non-small cell lung cancer admitted to Shanghai Chest Hospital was collected, and the serum of 27 normal people was also collected. Tumor classification was based on the WHO lung tumor classification standard, and pathological grading was based on the TNM standard. The clinical data of the patients were collected in the medical record room archives, including age, gender, tumor size, pathological analysis, and smoking history.

[0151] 2. Direct ELISA method to detect the content of FGF19 / β-klotho in serum, which specifically includes the following steps:

[0152] 1. Collect blood in a clean test tube, coagulate at room temperature for 30 minutes, centrifuge at 2000×g for 20 minutes, and collect serum. Analyze immediately or freeze at -20°C after aliquoting.

[0153] 2. Prepare 10,000pg / ml standard: Take 1ml of ...

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Abstract

The invention belongs to the field of tumor biomarkers and tumor diagnosis, and in particular relates to a non-small cell lung cancer detection kit and application thereof. The present invention finds for the first time that FGF19 and β-klotho can be used individually or in combination as biomarkers for early diagnosis, selection of treatment options, and prognosis assessment of non-small cell lung cancer, with high specificity and good sensitivity. It can realize the early in vitro diagnosis of non-small cell lung cancer, overcome the limitation that most of the original non-small cell lung cancer detection is biopsy detection, and overcome the shortcomings of the existing non-small cell lung cancer serum detection that is cumbersome and has many false positives.

Description

technical field [0001] The invention belongs to the field of tumor biomarkers and tumor diagnosis, and in particular relates to a non-small cell lung cancer detection kit and application thereof. Background technique [0002] Lung cancer can be divided into multiple tissue types according to different pathological characteristics. Different types of lung cancer have different treatment measures and prognosis effects. The histopathological classification of common lung cancer is divided into non-small cell carcinoma (NSCLC) and small cell carcinoma (SCLC). Non-small cell carcinoma is divided into squamous cell carcinoma (SCC), adenocarcinoma (AC) and large cell carcinoma (LCC), among which non-small cell lung cancer is the most common type of lung cancer, accounting for 80-85% of primary lung cancer . Once discovered, non-small cell lung cancer is generally in the middle and late stage, and the surgical treatment effect is not good. Therefore, the early diagnosis of non-sm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/577
Inventor 李凡夏伟梁李子明
Owner SHANGHAI JIAOTONG UNIV
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