Anti-African swine fever virus and anti-CD double-target pig-derived antibody, preparing method and application

A technology for the preparation of African swine fever virus and antibodies, which is applied in the field of anti-African swine fever virus and CD dual-target swine-derived antibodies, which can solve the problems of inability to fight against the infection and spread of African swine fever, the inability to treat African swine fever, and the inability to prevent it. question

Pending Publication Date: 2019-05-10
SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide an anti-African swine fever virus and CD dual-target swine-derived antibody, preparation method and application to solve the problem that the existing technology cannot fight against the infection and spread of African swine fever, resulting in the inability to prevent and treat African swine fever. swine fever problem

Method used

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  • Anti-African swine fever virus and anti-CD double-target pig-derived antibody, preparing method and application
  • Anti-African swine fever virus and anti-CD double-target pig-derived antibody, preparing method and application
  • Anti-African swine fever virus and anti-CD double-target pig-derived antibody, preparing method and application

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preparation example Construction

[0066] In the anti-African swine fever virus and CD dual-target porcine antibody preparation method provided by the present invention, it also includes:

[0067] Expanded culture: select high-expression stable transfected cell lines, ferment through step-by-step scale-up cell reactors, collect supernatant by filtration and remove cells and debris;

[0068]Antibody purification: the supernatant was captured by cation-exchange chromatography to capture the genotype or protein-type anti-ASFV and CD dual-target porcine antibody, further purified and refined by anion-exchange chromatography, and then passed ultra- The filter method is used for buffer replacement to produce high-purity genotype or protein-type anti-ASFV and CD dual-target porcine antibodies.

[0069] In the preparation method of the anti-African swine fever virus and CD dual-target porcine antibody provided by the present invention, the following operations are used to process the prepared anti-African swine fever v...

Embodiment 1

[0079] Example 1: Anti-African swine fever virus and CD dual-target porcine antibody binding titer detection on five representative antigenic proteins including VP73, P54, P72, A238L, and CD2V.

[0080] VP73, P54, P72, A238L, and CD2V were used as detection antigens respectively, and the antibody titers of the anti-ASFV and CD dual-target swine-derived antibodies prepared by "ELISA" method (enzyme-linked immunoassay) were detected, and the results As shown in the table below:

[0081]

[0082] Note: The concentration of the anti-ASF virus and CD dual-target porcine antibody solution in the test sample is 1 mg / mL.

[0083] It can be seen from the above test results that the prepared anti-ASFV and CD dual-target porcine antibodies have high antibody binding titers to representative VP73, P54, P72, A238L, and CD2V protein antigens.

Embodiment 2

[0084] Example 2: Anti-African swine fever virus and CD dual-target porcine antibody binding titer detection for CD. Take the CD3 antigen as an example.

[0085] Using CD3 as the detection antigen, the antibody titer of the prepared anti-ASFV and CD dual-target porcine antibody was detected by "ELISA" method (enzyme-linked immunosorbent assay), and the results are shown in the following table:

[0086]

[0087] Note: Anti-African swine fever virus and CD dual-target porcine antibodies in test samples

[0088] The solution concentration is 1 mg / mL.

[0089] It can be seen from the above test results that the prepared anti-ASFV and CD dual-target porcine antibody has a high antibody binding titer to CD3 antigen.

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Abstract

The invention relates to the technical field of biological medicine, in particular to an anti-African swine fever virus and anti-CD double-target pig-derived antibody, a preparing method and application. The prepared anti-African swine fever virus and anti-CD pig-derived double-target compound antibody has two antigen combination sites which are combined with African swine fever viruses and immunecells respectively to generate the bridging effect and realize the two major functions; on one hand, the antigen proteins which cause immunosuppression and immune escape are inactivated, the immunosuppression and immune escape are eliminated, and the African swine fever viruses entering a body are killed; on the other hand, the functions of T cells are activated, the immune system is restored, the cell-mediated immunity is started, and virus expansion is prevented; meanwhile, infected cells are split and dissolved, phagocytes are activated to clear away the viruses and the infected cells, andthe aim of preventing and treating the African swine fever viruses is achieved. By means of the compound antibody, a biological medicine and a biological safety feed additive which eliminate the infection of the African swine fever viruses and prevent and treat the African swine fever can be prepared.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, and more specifically relates to an anti-African swine fever virus and CD dual-target swine-derived antibody, a preparation method and an application. Background technique [0002] African swine fever (African swine fever, ASF) is an acute and severe infectious disease caused by African swine fever virus (ASFV). Not only seriously endanger the pig industry in my country and the world, but also seriously affect the international trade related to the pig industry and other related industries. The World Organization for Animal Health (Office international desépizooties, OIE) lists ASF as a must-report animal disease, and our country lists it as a first-class animal disease, which is an exotic disease that is focused on prevention and control. [0003] Given that African swine fever is a complex disease that spreads rapidly around and invades the food chain causing major socio-economic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/46A61K39/42A61K39/395A61P31/20A01N61/00A01P1/00A23K20/195
Inventor 包晟杨荣鉴王长安廖列扬曹天福龚芹
Owner SHENZHEN JASON INTELLIGENT BIOTECH CO LIMLTED PRC
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