Chemical treatment method for micro-fluidic chip application protein detection
A protein detection and microfluidic chip technology, applied in the field of biomedicine, can solve the problems of insufficient detection accuracy, large solid-phase carrier detection area, uneven cTnI capture, etc., to achieve small detection area, uniform capture, and detection high precision effect
Inactive Publication Date: 2019-05-17
江苏医联生物科技有限公司
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Problems solved by technology
Traditional protein chips generally carry out special chemical treatment on the whole solid phase carrier, and then immobilize known protein molecules on it to capture the target protein that can specifically bind to it, so as to realize the detection and analysis of biomolecules. However, traditional protein chips There are the following defects: the detection area of the solid phase carrier is large, the capture of cTnI is uneven, and the detection accuracy is not high enough
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[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0028] A chemical processing method for protein detection applied to a microfluidic chip, comprising:
[0029] Step 1: Apply BS to the surface of the glass sheet 3 solution for processing; figure 1 shown, including:
[0030] Step 1) using acetone, isopropanol and deionized water to ultrasonically clean the glass sheet sequentially for 4 to 6 minutes, preferably 5 minutes;
[0031] Step 2)...
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The invention relates to a chemical treatment method for micro-fluidic chip application protein detection. The method comprises the following steps: step one, treating a glass sheet surface by using BS3 solution; step two, performing ELISA test on a glass carrier bound with BS3 on the surface ; the step one comprises the following operations: ultrasonically cleaning the glass sheet by using acetone, isopropanol and deionized water in order; blow-drying the glass sheet by using the nitrogen, and performing plasma treatment on the glass sheet surface, and adding APTMS solution on the glass sheetsurface, and reacting at the room temperature; cleaning and air-drying the glass sheet, heating the glass sheet, thereby realizing surface bonding; adding BS3 solution on the glass sheet surface after recovering the room temperature to obtain the glass carrier bounded with the BS3 on the surface. Through the chemical treatment method for micro-fluidic chip application protein detection disclosedby the invention, the obtained solid-phase carrier detection region is small in area, the capturing on the cTnI is uniform, the detection precision is high, the powerful technical support can be provided for the antigen and antibody detection and medicine screening and like fields, and the demand of the actual application can be well satisfied.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a chemical processing method for protein detection applied in a microfluidic chip. Background technique [0002] The protein chip is a dot array hybridization chip with zero microflow, which belongs to a special type of microfluidic chip. Microfluidic chip technology is a technology that integrates basic operating units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes on a micron-scale chip. Due to its great potential in the fields of biology, chemistry, and medicine, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, and machinery. Traditional protein chips generally carry out special chemical treatment on the whole solid phase carrier, and then immobilize known protein molecules on it to capture the target protein that...
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IPC IPC(8): G01N33/53G01N33/531G01N33/533G01N33/543G01N33/577G01N33/68
Inventor 杨文婷曹臻陈亮
Owner 江苏医联生物科技有限公司