Induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into osteoblasts

A technology of osteoblast differentiation and mesenchymal stem cells, applied in the field of biomedicine, can solve problems such as difficulty in obtaining a large number of bone marrow cells and source limitations, and achieve good cryopreservation effect, improved survival rate, and high expression rate

Inactive Publication Date: 2019-05-21
和携科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] MSCs were originally found in bone marrow. Because the collection of bone marrow-derived MSCs requires bone marrow aspiration, the source is limited, and it is very difficult to obtain a large number of bone marrow cells.

Method used

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  • Induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into osteoblasts

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Embodiment 1

[0025] A method for promoting the differentiation of human umbilical cord mesenchymal stem cells into osteoblasts, comprising the following steps: including the step of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells, and the step of differentiation of stem cells into chondrocytes induction step.

[0026] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of umbilical...

Embodiment 2

[0031] A method for promoting the differentiation of human umbilical cord mesenchymal stem cells into osteoblasts, comprising the following steps: including the step of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells, the step of cryopreservation of stem cells, The step of recovering and culturing stem cells and the step of inducing the differentiation of stem cells into chondrocytes.

[0032] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical...

Embodiment 3

[0044] A method for promoting the differentiation of human umbilical cord mesenchymal stem cells into osteoblasts, comprising the following steps: including the step of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells, and the step of differentiation of stem cells into chondrocytes induction step.

[0045] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of umbilical...

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Abstract

The invention relates to an induction method for promoting differentiation of human umbilical cord mesenchymal stem cells to osteoblasts, which comprises the following steps of: acquiring umbilical cord tissue blocks, carrying out primary culture on the stem cells, carrying out subculture on the stem cells and induction differentiation of the stem cells to chondrocytes, wherein the induction of the differentiation of the stem cells to the chondrocytes comprises the following steps of: carrying out resuspension subculture on a serum-free culture medium until Pe generation human umbilical cord mesenchymal stem cells are prepared into a Pe generation human umbilical cord mesenchymal stem cell suspension solution; the Pe generation human umbilical cord mesenchymal stem cell suspension solutionis cultured in serum-free medium containing an osteogenic differentiation inducer, the serum-free medium containing the osteogenic differentiation inducer is replaced every three and a half days, after the induction is carried out for 14 days, osteoblasts are obtained; wherein e=6, 7, 8, 9, 10. The method can differentiate human umbilical cord mesenchymal stem cells into osteoblasts, and has great significance in repairing human bone tissues and treating bone injuries.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an induction method for promoting the differentiation of human umbilical cord mesenchymal stem cells into osteoblasts. Background technique [0002] Avascular necrosis of the femoral head is a disease caused by insufficient blood supply due to trauma, rheumatism, etc., which causes pathological changes in the femoral head. , life can not take care of themselves. Western medicine mainly adopts the treatment of replacing the femoral head, which can relieve symptoms, but the use time is limited. [0003] The emergence of mesenchymal stem cells (MSCs) has brought hope for the repair and treatment of osteogenic injuries. Mesenchymal stem cells are an important member of the stem cell family, a type of pluripotent stem cells derived from the mesoderm and ectoderm in the early development, with self-renewal, high proliferation and multi-directional differentiation potential. Unde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
Inventor 赵进军谷广其杨桂花赵宇飞李张鹏
Owner 和携科技有限公司
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