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Method for preparing human umbilical cord mesenchymal stem cell injection for treating ischemic femoral head necrosis by cavity injection

A technology of femoral head necrosis and mesenchymal stem cells, which is applied in the field of biomedicine, can solve the problems of difficulty in obtaining a large number of bone marrow cells and source limitations, and achieve good cryopreservation effects, perfect reserves, and guaranteed survival rates

Inactive Publication Date: 2019-06-14
和携科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] MSCs were originally found in bone marrow. Because the collection of bone marrow-derived MSCs requires bone marrow aspiration, the source is limited, and it is very difficult to obtain a large number of bone marrow cells.

Method used

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  • Method for preparing human umbilical cord mesenchymal stem cell injection for treating ischemic femoral head necrosis by cavity injection

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Embodiment 1

[0023] A preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection treatment of ischemic femoral head necrosis, comprising the steps of obtaining umbilical cord tissue block, the step of primary culture of stem cells, the step of subculture of stem cells and human Preparation steps of source umbilical cord mesenchymal stem cell injection.

[0024] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of...

Embodiment 2

[0029] A preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection treatment of ischemic femoral head necrosis, comprising the steps of obtaining umbilical cord tissue block, the step of primary culture of stem cells, the step of subculture of stem cells, the step of stem cell The cryopreservation steps, the recovery and culture steps of stem cells and the preparation steps of human umbilical cord mesenchymal stem cell injection.

[0030] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat ...

Embodiment 3

[0042] A preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection treatment of ischemic femoral head necrosis, comprising the steps of obtaining umbilical cord tissue block, the step of primary culture of stem cells, the step of subculture of stem cells and human Preparation steps of source umbilical cord mesenchymal stem cell injection.

[0043]The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of ...

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Abstract

The invention relates to a method for preparing a human umbilical cord mesenchymal stem cell injection for treating ischemic femoral head necrosis by cavity injection. The method comprises the steps of obtaining an umbilical tissue block, performing primary culture of stem cells, subculturing the stem cells, and preparing the human umbilical cord mesenchymal stem cell injection. The method for preparing the human umbilical cord mesenchymal stem cell injection comprises resuspending the Pe-generation human umbilical cord mesenchymal stem cells with a compound electrolyte injection, and repeatedly purging; after the Pe-generation human umbilical cord mesenchymal stem cell suspension is uniform, supplementing the compound electrolyte injection to adjust the concentration of the human umbilical cord mesenchymal stem cells to 4*10<4-6>*10<4> cells / mL, and adding an osteogenic differentiation inducer, to obtain the human umbilical cord mesenchymal stem cell injection, wherein e is equal to 6, 7, 8, 9, and 10. The human umbilical cord mesenchymal stem cells can be differentiated into osteoblasts, and the method has important significance in repairing human bone formation tissue and treating bone damage.

Description

technical field [0001] The invention relates to the technical field of biomedicine, more specifically, it relates to a preparation method of human umbilical cord mesenchymal stem cell injection for lacunar injection treatment of ischemic femoral head necrosis. Background technique [0002] Avascular necrosis of the femoral head is a disease caused by insufficient blood supply due to trauma, rheumatism, etc., which causes pathological changes in the femoral head. , life can not take care of themselves. Western medicine mainly adopts the treatment of replacing the femoral head, which can relieve symptoms, but the use time is limited. [0003] The emergence of mesenchymal stem cells (MSCs) has brought hope for the repair and treatment of osteogenic injuries. Mesenchymal stem cells are an important member of the stem cell family, a type of pluripotent stem cells derived from the mesoderm and ectoderm in the early development, with self-renewal, high proliferation and multi-dir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K38/18A61K9/08A61P19/08C12N5/0775A01N1/02A61K31/352A61K31/661A61K31/375A61K31/573
Inventor 赵进军谷广其杨桂花赵宇飞李张鹏
Owner 和携科技有限公司
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