Blood cell counting device and method based on rated number

Abstract

The invention discloses a blood cell counting device and method based on rated number. The device comprises an impedance cell detection device, a control circuit, an electromagnetic valve, a trace flowmeter, a timing starting unit and a waste liquid pump and is characterized in that the electromagnetic valve, the trace flowmeter, the timing starting unit and the waste liquid pump are sequentiallyconnected through guide pipes, the liquid inlet end of the electromagnetic valve is connected to a rear reaction chamber through a guide pipe, and the impedance cell detection device, the electromagnetic valve, the trace flowmeter, the timing starting unit and the waste liquid pump are connected with the control circuit. The blood cell counting device and method based on rated number has the advantages that the rated number of cells participating in the counting can be fixed at a large value, the volume of blood cell diluent passing micropores can be calculated by multiplying counting time bythe measured value of the trace flowmeter in the counting time, a volume setting device is omitted, a liquid path system can be simplified, structure space can be reduced, blood cells in in content in blood can be measured to obtain a large cell number, and instrument measuring precision can be increased.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a blood cell counting device and method based on a rated number. Background technique [0002] In recent years, with the advancement of science and technology and the widespread use of computers, a large number of automated instruments have been introduced into blood testing. Blood analyzers are one of the instruments widely used in hospital clinical testing. It makes the processing of blood cell count simpler, faster and more accurate, effectively reduces the work intensity of medical staff, and improves work efficiency. [0003] Currently, most blood analyzers use the electrical impedance principle (Coulter principle) to detect blood cells. On the premise of using the principle of electrical impedance to count blood cells, an important parameter to measure the performance of the blood analyzer is the CV value measured by the instrument for blood cells. The lower the CV...

AI Technical Summary

Problems solved by technology

Because the counting method is simple and fast, it meets the needs of blood analyzers for counting blood cells to a certain extent, but there are also some shortcomings: ① When the hemodilution ratio is too low and the blood cell concentration is high, not only the blood sample is wasted, At the same time, the frequency of blood cell counting is too high, which causes certain difficulties in circuit design and signal sampling.

Excessive blood cell concentration is more likely to cause blockage of the micropores of the electrical impedance detection device

②When the blood dilution ratio is too high and the blood cell concentration is low, the number of cells measured in the rated volume will be low, which will have a great impact on the CV value of the instrument and affect the doctor's judgment

At the same time, for cells with a small content in blood, if the detection accuracy is to be improved, a larger constant volume device is required, so the application range of this method to the hemodilution ratio is narrow

③ Due to the large number of cells, under the rated volume, the detection deviation of micro volume due to objective reasons such as vibration will cause a large change in the detection value of the cell, reducing the detection accuracy of the instrument

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