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Automated body fluid analysis

A body fluid, automatic system technology, applied in the direction of analyzing materials, image analysis, image enhancement, etc., can solve problems such as large signal changes

Inactive Publication Date: 2019-07-02
ABBOTT LAB INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Other factors that can adversely affect the quality of flow cytometry data include variations in nucleated cell counts from sample to sample, which, for example, lead to variations in fluorescent signals such that signals related to cellular events vary widely among different types of samples. Big

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[0030] definition

[0031] The phrases "watershed transform" or "watershed algorithm" are used interchangeably and reference is made to Luc Vincent and Pierre Soillie, Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Transactions on Pattern Analysis and Machine Intelligence, 13(6), 1991, pp .583-598.

[0032]As used herein, the term "body fluid" generally refers to a fluid derived from a "biological sample," which includes a variety of sample types obtained from an individual or population of individuals, and which can be used in diagnostic, monitoring, or screening assays. This definition includes blood and other fluid samples of biological origin. This definition also includes samples that have been manipulated in any way after procurement, such as by mixing or pooling individual samples, treating with reagents, lysing, or enriching for certain components, such as nucleated cells, non-nucleated cells, pathogens, etc.

[0033] The ...

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Abstract

Methods, devices, and systems for automated cellular analysis of a body fluid sample are disclosed. The methods, devices, and systems apply watershed transform to data, generated by flowing a body fluid sample through a flow cytometer, to determine threshold(s) to be used for analysis of the data.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 385,055, filed September 8, 2016, which is hereby incorporated by reference in its entirety. Background technique [0003] Analysis of body fluids provides important information about the composition of body fluids, including cell types, counts of different types of cells, presence of abnormal cells, presence of infectious particles (eg, prions, viruses, bacteria, etc.). This information is critical to provide clinical diagnosis and prognosis. [0004] A variety of methods are used to analyze cells in various biological fluid samples. Methods for cell analysis include visual and / or automated inspection by light or fluorescence microscopy. Cytometry and these types of analyzes are typically performed to obtain information about the lineage, maturation stage, and / or cell count of the cells in the sample. [0005] Analyzing cells in biologi...

Claims

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Application Information

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IPC IPC(8): C12M1/34
CPCG01N2015/1006G01N2015/1402G01N15/1459G01N2015/1488G06T7/11G06T7/136G06T7/155G06T2207/20152G06T2207/30024G06T2207/10056G06V20/695G06V20/698G06V10/267G01N33/5002G01N33/52G01N33/5094
Inventor W·李E·H·林吴炯
Owner ABBOTT LAB INC
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