Automatic operating system and carrier device for cell vitrification

Abstract

The present invention provides an automatic operating system for cell vitrification. The system comprises a console device, including a storage mechanism for storing a carrier device, a console mechanism configured to position the carrier device of a tray at a standard station, and a transport mechanism used for transporting the carrier device to the console mechanism; a mechanical arm device including an suspension arm and a mechanical arm disposed below the suspension arm; a liquid drop changing device including a bracket, a movable pipe body receiver, a liquid drop feeding pipe and liquid drop suction pipe, with the liquid drop feeding pipe and the liquid drop suction pipe being connected to the pipe body receiver and being above a liquid drop changing zone; a target cell liquid transfer device comprising a moving frame, a pipette for sucking the target cell liquid, and a pump body for providing a suction force for the pipette; a master controller controlling operation of the console device, the mechanical arm device, the liquid drop changing device and the target cell liquid transfer device. The system can allow vitrification of living cells to be rapidly and effectively achieved.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation and resuscitation of human tissue, and in particular relates to an automatic operating system and a carrying device for cell vitrification. Background technique [0002] The basic principle of vitrification is to solidify a high concentration of cryoprotectant in an ultra-low temperature environment to form an irregular vitrified solid, which preserves the normal distribution of molecules and ions in the liquid state, so it can play a role in vitrification in cells. to the protective effect. The current freezing technology can be used to cryopreserve sperm, eggs and embryos at different stages, especially the vitrification technology, which has not only been widely recognized by the medical field, but also gradually replaced the traditional slow freezing method, and has become the most advanced freezing technology. good choice. [0003] Most of the existing technologies are mainly manua...

AI Technical Summary

Problems solved by technology

This manual operation method requires the time to be as short as possible and the coating surface to be as thin as possible to cool down quickly; this operation often requires the operator to have skilled operating skills, and the operator is required to set the droplet into a gradient concentration solution, so that the cells in the gradient This process requires rapid operation. Improper operation may lead to abnormal freezing of living cells due to excessive osmotic pressure or inability to standardize the operation process, and the cells are easily dead or lost.

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