Dopamine detection sensor and detection method based on electrochemiluminescence energy transfer
A technology for detecting sensors and luminous energy, which is applied in the fields of electrical excitation analysis, material excitation analysis, etc., to achieve the effects of high sensitivity, strong anti-interference, and reduced background signal
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[0038] The preparation method of the sensor specifically includes the following steps:
[0039] 1) Preparation of H-g-C 3 N 4 (alkaline hydrolysis g-C 3 N 4 );
[0040] 2) Preparation of CNT-AuNP;
[0041] 3) preparing gold nanoclusters;
[0042] 4) Electrode modification: sequentially modify CNT-AuNP and H-g-C on the electrode surface 3 N 4 Finally, gold nanoclusters were modified on the electrode surface to obtain a sensor for detecting dopamine.
[0043] Wherein, the step 4) includes: first drop CNT-AuNP on the electrode surface after pretreatment, drop H-g-C 3 N 4 , after drying, add EDC / NHS dropwise, and then add amino-modified DNA-1 dropwise, so that DNA-1 is combined with the electrode through an amide bond, and finally add gold nanoclusters connected with DNA-2, wherein, DNA-1 and DNA -2 can be paired complementary.
Embodiment 1
[0045] 1) Preparation of H-g-C 3 N 4 : 30mg g-C 3 N 4 and 2.5g NaOH were dissolved in 25mL of water, sonicated for 1.5h to fully disperse, then stirred overnight at 65°C, then added 1.0gNaOH, and sonicated for 2h; the obtained solution was transferred to a dialysis bag (Mw=1000), and the solution The dialysis lasts for 48 hours, and the water is changed every 12 hours to remove NaOH. After completion, the inner liquid is transferred to a centrifuge tube, and centrifuged at 6500rpm for 15 minutes to remove particles and large sheets, and retain the supernatant to obtain H-g-C 3 N 4 aqueous solution.
[0046] 2) Preparation of CNT-AuNP (carbon nanotube composite gold nanoparticles):
[0047] 2-1) Prepare colloidal AuNPs (the diameter is 13nm in this example) with chloroauric acid solution and sodium citrate as raw materials: 96mL H 2 O and 4 mL of 1% HAuCl 4 Mix and stir the solution and heat to boiling, quickly add 10mL 0.1141g sodium citrate, boil for 15min, then cool a...
Embodiment 2
[0065] A dopamine detection method based on electrochemiluminescence energy transfer, using the sensor prepared in Example 1 for dopamine detection, combined with electrochemical analysis means to obtain the detection result.
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