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Method for measuring trace alkaloids and nitrosamines in mainstream smoke of cigarettes

A technology for cigarette mainstream smoke and measurement methods, which is applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as complex processing processes, achieve the effects of reducing impurity interference, reducing matrix interference, and improving accuracy

Pending Publication Date: 2019-07-05
CHINA TOBACCO GUIZHOU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods for the determination of tobacco-specific nitrosamines in mainstream cigarette smoke mainly include gas phase-thermal energy analyzer method and liquid chromatography-tandem mass spectrometry, etc. The sample pretreatment process of the method is often complicated

Method used

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  • Method for measuring trace alkaloids and nitrosamines in mainstream smoke of cigarettes
  • Method for measuring trace alkaloids and nitrosamines in mainstream smoke of cigarettes
  • Method for measuring trace alkaloids and nitrosamines in mainstream smoke of cigarettes

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Experimental program
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Effect test

Embodiment 1

[0037] Such as Figure 11 As shown, the determination of trace alkaloids and nitrosamines in mainstream cigarette smoke of the present embodiment comprises:

[0038] 1) Collection of total particulate matter in mainstream cigarette smoke

[0039] At a temperature of 22±2°C and a relative humidity of (60±5)%, use a linear smoking machine to smoke 5 cigarettes according to the ISO suction conditions (puffing interval 60s, duration 2s, suction volume 35mL) Cigarettes, and the total particulate matter in the mainstream smoke of 3 cigarettes was collected using Cambridge filters, labeled as Cigarette 1, Cigarette 2 and Cigarette 3.

[0040] 2) Sample pretreatment

[0041] Put the Cambridge filter in a 50mL Erlenmeyer flask, add 200μL internal standard in turn to accelerate solvent extraction at room temperature for 10min, after standing for a while, take about 1.5mL supernatant and add it to a 2mL centrifuge tube containing QuECHERS reagent, Vortex and mix for 2 minutes, centrif...

Embodiment 2

[0050] Embodiment 2 methodological investigation

[0051] 1) Standard curve

[0052] Using acetonitrile as solvent, a series of standard solutions of different concentrations of trace alkaloids and tobacco-specific nitrosamines were prepared, and the above instrument conditions were used for UPLC-HR-MS analysis. Then adopt the internal standard method to quantify (internal standard is: mesmin-D4, cotinine-D3 and nicotriptyline-D9), with the peak area ratio of each target and its internal standard as the ordinate (Y), each target The abscissa concentration (X, ng / mL) was used for regression analysis to obtain 6 kinds of trace alkaloids (Pseudine, Nornicotine, Mesmin, Cotinine, Nicotriptyline and N-formyl Nornicotine) Nicotine), the regression equations and their correlation coefficients of four tobacco-specific nitrosamines (NNK, NNN, NAB and NAT), as shown in Table 2.

[0053] It can be seen from Table 2 that the linear relationship of the standard curves of 6 kinds of trace...

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Abstract

The invention provides a method for measuring trace alkaloids and nitrosamines in the mainstream smoke of cigarettes. The method includes the following steps that: a) cigarettes are smoked, total particulate matters in the mainstream smoke of the cigarettes are collected, and are added into to an extraction solvent and a deuterated isotope internal standard so as to be subjected to extraction, anextract liquid is collected; b) the extract liquid and a QuECHERS reagent are subjected to vortex mixing for 0.5-10 min, centrifuging is performed at a speed of 9000-11000 rpm for 3-7 min, so that a supernate can be obtained; and c) a UPLC-HR-MS method is adopted to perform quantitative analysis on trace alkaloids and specific nitrosamines for the cigarettes in the supernate. With the method disclosed by the invention adopted, 6 trace alkaloids, namely, neonicotine, nornicotine, myosmine, cotinine, nicotelline and N-formyl nornicotine and four specific nitrosamines, namely NNN, NNK, NAT and NAB in the main stream smoke of the cigarettes can be measured simultaneously. The method has the advantages of high sensitivity, a high accuracy and high selectivity, and is suitable for the rapid determination of trace alkaloids and nitrosamines in batch cigarette filters.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing of chemical components of tobacco products, and in particular relates to a method for measuring trace alkaloids and nitrosamines in mainstream smoke of cigarettes. Background technique [0002] Trace alkaloids (such as pseudobaitine, nornicotine, mesmin, cotinine, nicotine, and N-formyl nornicotine) are one of the most important chemical components in tobacco and cigarette smoke. The content of trace alkaloids in tobacco has an important impact on the internal quality, safety and usability of tobacco leaves. There is a significant positive correlation between trace alkaloids and aroma, concentration, miscellaneous gas, and irritation. Pseudomine in trace alkaloids , Anonicotinoid and aftertaste were significantly negatively correlated. The trace alkaloids anatabine and anatabine in tobacco can be converted into N-nitroso anatabine (NAB) and N-nitroso anatabine (NAT) respecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 张小涛刘剑张丽阮艺斌王维维姬厚伟万强
Owner CHINA TOBACCO GUIZHOU IND
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