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Human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere

An environmental chamber and air technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of lack of visual observation of gamete freezing and thawing, achieve rapid and controlled rewarming, and avoid bacterial viruses or Impurity contamination, effects of high thermal conductivity

Active Publication Date: 2019-07-12
上海艾尔温生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this patent, the gamete carrier is placed in a liquid nitrogen fumigation box without an observation window, let alone the conditions for visually observing the gamete freeze-thaw phenomenon

Method used

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  • Human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere
  • Human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere
  • Human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere

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Embodiment Construction

[0024] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0025] see Figure 1 to Figure 3 , as can be seen from the figure, the human gamete rapid freezing and thawing visualization environment chamber of the present invention maintains atmospheric air atmosphere, including the environment chamber main body 1, the high thermal conductivity bottom plate 2, the upper flange cover plate 3, the O-shaped washer 4, the pre-tightening screw 5, The capillary 6, the sterilizing dryer 7, the thermocouple 8 and the electric heating film 9, the main body 1 of the environmental chamber is a flat hollow cylindrical structure, which together with the high thermal conductivity bottom plate 2 and the upper flange cover plate 3 form a closed Cavity; the outer side of the main body 1 of the environmental chamber is provided with a capillary interface 10, a thermocouple penetrating interface 11, an electric heating film...

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Abstract

The invention relates to a human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere. The human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere mainly comprises an environment cabin main body, a high heat conduction bottom plate, an upper flange cover plate, an O-shaped gasket, a pre-tightening screw, a capillary tube, a sterilization dryer, a thermocouple and an electric heating membrane, wherein the environment cabin main body is of a flat hollow cylindricalstructure; and a closed cavity is formed by the environment cabin main body, the high heat conduction bottom plate and the upper flange cover plate. High-transmittance quartz glass is embedded in thecenter of the upper flange cover plate and is buckled to the top surface of the environment cabin main body, and a capillary tube connector, a thermocouple cabin penetrating connector, an electric heating membrane cabin penetrating connector and a connecting rod are arranged on a side surface of the environment cabin main body. Rapid freezing and thawing of a human gamete sample under the condition of constant atmospheric pressure can be realized. The human gamete rapid freezing and thawing visual environment cabin capable of maintaining atmospheric pressure air atmosphere has a visual condition in the whole process and real-time temperature measurement capability, and is suitable for observing a transient vitrification or crystallization freezing and thawing process of a gamete in a low-temperature cooling or rewarming process.

Description

technical field [0001] The invention relates to a visual environment chamber for rapid freezing and thawing of human gametes that maintains a normal-pressure air atmosphere, in particular to a chamber capable of repeatedly loading gamete samples, enabling the samples to be in spaced contact with a low-temperature cold surface but rapidly cooling down, and ensuring that there is always a Oxygen normal pressure environment and internal high-transparency visible device. Background technique [0002] Vitrification freezing technology has gradually become one of the important application technologies in assisted reproduction technology. Ice crystals to avoid physical damage to cells. The vitrification cryopreservation method has the advantages of short operation time, simplicity, low demand for equipment, and good cryopreservation effect. In order to be able to repeatedly load gamete samples, to keep the samples in contact with the low-temperature cold surface at intervals but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0257
Inventor 黄永华吴栋梁张宏彬李铮姚晨成李朋
Owner 上海艾尔温生命科技有限公司
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