Method for measuring contents of vitamin D by two-dimensional column switching high performance liquid chromatography

A high-performance liquid chromatography and liquid chromatography technology, which is applied in the field of two-dimensional column switching high-performance liquid chromatography to determine vitamin D content, can solve the problem of difficulty in determining the accuracy and reproducibility of the measurement results, and the cumbersome and complicated experimental process, etc. problem, to achieve the effect of simplifying the pre-processing process, shortening the experiment time, and reducing human error

Active Publication Date: 2019-07-19
福建省食品药品质量检验研究院 +1
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Problems solved by technology

Especially for the determination of vitamin D content in pharmaceutical vegetable oil preparations (that is, the 0722 vitamin D determination method in the current CHP2015 edition), the entire experimental process is cumbersome and complicated, requiring multiple transfers and reconstitution, and a very small amount of solvent is used, which leads to It is difficult to grasp the accuracy and reproducibility of

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  • Method for measuring contents of vitamin D by two-dimensional column switching high performance liquid chromatography
  • Method for measuring contents of vitamin D by two-dimensional column switching high performance liquid chromatography
  • Method for measuring contents of vitamin D by two-dimensional column switching high performance liquid chromatography

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Embodiment Construction

[0028] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0029] 1. Instruments and reagents

[0030] (1) Materials and reagents

[0031] Vitamin D drops, vitamin AD drops, cod liver oil (the sample is commercially available); n-hexane, isopropanol, isooctane (chromatographically pure, Merck, Germany); n-pentanol (high-grade pure, Aladdin); vitamin D 3 Reference substance (batch number: 100061-201208, provided by China National Institutes for Food and Drug Control, purity 99.8%).

[0032] (2) Instruments

[0033] Dual ternary high performance liquid chromatography (ThermoFisher U3000 DGLC), equipped with dual ternary chromatographic pumps, DAD detector, ultraviolet detector, column thermostat (with 2 position ten-way valve, 9ml collection tube); 100,000 points One of the analyt...

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Abstract

The invention discloses a method for measuring the contents of vitamin D by two-dimensional column switching high performance liquid chromatography. Secondary switching normal-phase chromatography isadopted for detection, provitamin D and vitamin D in a sample can be simultaneously measured, in addition, after a sample to be measured is dissolved by normal hexane, the sample to be measured can bedirectly measured so as to avoid a complex sample pretreatment process, and therefore, the method is suitable for the accurate detection of trace vitamin D in plant oil preparation.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to a method for measuring vitamin D content by two-dimensional column switching high-performance liquid chromatography. Background technique [0002] After reviewing the five major pharmacopoeias at home and abroad, the vitamin D determination method is only recorded in the CHP2015 edition and USP41. In addition to the vitamin D content determination method recorded in the Pharmacopoeia, two determination methods are also recorded in food. However, a comparison of various vitamin D content determination methods at the present stage shows that different degrees of pretreatment are required when determining the trace vitamin D content in samples. Especially for the determination of vitamin D content in pharmaceutical vegetable oil preparations (that is, the 0722 vitamin D determination method in the current CHP2015 edition), the entire experimental process is cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 林思荣王勇修虹陈鼎雄许永彬林晨楼永明许福春陈喜生苏志伟喻喜华曾惠兰石贞香王一
Owner 福建省食品药品质量检验研究院
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