Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A New Method for Detection of Immune Complexes by Size-Exclusion Chromatography

An immune complex and size-exclusion chromatography technology, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of low sensitivity and inability to detect detection objects, achieve wide application range, shorten detection time, save Effect of washing steps

Active Publication Date: 2022-06-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it judges the result by observing the agglutination reaction with the naked eye, the sensitivity is not high, and it cannot detect the detection objects that require high sensitivity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A New Method for Detection of Immune Complexes by Size-Exclusion Chromatography
  • A New Method for Detection of Immune Complexes by Size-Exclusion Chromatography
  • A New Method for Detection of Immune Complexes by Size-Exclusion Chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] see image 3 , provides a kind of exclusion chromatography detection method of hepatitis B surface antigen, and the steps are as follows:

[0045] (1) coating the surface of the carrier particles with hepatitis B surface antibody, and labeling the paired hepatitis B surface antibody with fluorescein;

[0046] (2) Add the sample 1 to be tested, the carrier particles 2 coated with the hepatitis B surface antibody and the fluorescein-labeled hepatitis B surface antibody 3 into the upper column 20 at the same time, and incubate at 37°C for 1 hour;

[0047] (3) Centrifugation, so that the carrier particles pass through the molecular sieve chromatography medium 4 to the bottom of the lower end small column 22, and the result is interpreted by an instrument (such as a fluorescence microscope). If the surface of the carrier particles at the bottom of the lower small column 22 has fluorescein, it is positive 5; if there is no fluorescein on the surface of the carrier particles at...

Embodiment 2

[0049] see Figure 4 , a kind of exclusion chromatography detection method of syphilis antibody is provided, and the steps are as follows:

[0050] (1) Coat the surface of the carrier particles with syphilis antigens, and label the paired syphilis antigens with quantum dots;

[0051] (2) adding the sample 1 to be tested, the carrier particles 2 coated with the syphilis antigen and the syphilis antigen 3 labeled with quantum dots into the upper column 20 at the same time, and incubating at 37°C for 1 hour;

[0052] (3) Centrifugation, so that the carrier particles pass through the molecular sieve chromatography medium 4 to the bottom of the small column 22 at the lower end, and the result is interpreted by an instrument (such as an ultraviolet imager). If there are quantum dots on the surface of the carrier particles at the bottom of the lower end small column 22, it is positive 5; if there is no quantum dot on the surface of the carrier particle at the bottom of the lower end...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new method for detecting immune complexes by size-exclusion chromatography, which adopts a double-antigen or double-antibody sandwich method for detection, and specifically includes the following steps: 1) selecting carrier particles, and coating the surface of the carrier particles with detection antigen / Antibody, label the paired antigen / antibody with a marker; 2) Add the carrier particles coated with the detection antigen / antibody, the antigen / antibody with the marker, and the sample to be tested into the reaction container at the same time; 3) Incubation and centrifugation; 4) Detected by a detector. Compared with traditional ELISA and CLIA, the new method of size exclusion chromatography detection of immune complexes of the present invention saves washing steps, is easy to operate, and shortens the detection time; antigen and antibody belong to the same phase reaction in the microcolumn cavity, and the reaction is sufficient , to avoid the shortcomings of insufficient heterogeneous reaction of ELISA; compared with MGIA, the use of fluorescent and other immunolabeling assays amplifies the signal, greatly improves the sensitivity, and has a wider range of applications.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a new method for detecting immune complexes by exclusion chromatography. Background technique [0002] Immunodiagnosis is a method for detecting various diseases based on the specific immune response between antigens and antibodies. Immune complex, also known as antigen-antibody complex, is the product of the combination of antigen and antibody, and is the detection object of immunodiagnosis. Usually, a known antibody or antigen is labeled with a substance that is easy to display, and the presence or absence of the antigen-antibody reaction is reflected by detecting the label, thereby indirectly detecting a trace amount of antigen or antibody. Commonly used labels include enzymes, fluorescein, radioisotopes, colloidal gold, and electron-dense substances. The specific reaction of the display on this antigen or antibody label is called immunolabeling technology. [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/559
CPCG01N33/559
Inventor 李勇段生宝丁少华陈晔洲王红梅魏双施谢劲松李冬李剑平刘永茂
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com