31results about How to "Eliminates washing steps" patented technology

The invention discloses a new method for detecting immune complexes by size-exclusion chromatography, which adopts a double-antigen or double-antibody sandwich method for detection, and specifically includes the following steps: 1) selecting carrier particles, and coating the surface of the carrier particles with detection antigen / Antibody, label the paired antigen / antibody with a marker; 2) Add the carrier particles coated with the detection antigen / antibody, the antigen / antibody with the marker, and the sample to be tested into the reaction container at the same time; 3) Incubation and centrifugation; 4) Detected by a detector. Compared with traditional ELISA and CLIA, the new method of size exclusion chromatography detection of immune complexes of the present invention saves washing steps, is easy to operate, and shortens the detection time; antigen and antibody belong to the same phase reaction in the microcolumn cavity, and the reaction is sufficient , to avoid the shortcomings of insufficient heterogeneous reaction of ELISA; compared with MGIA, the use of fluorescent and other immunolabeling assays amplifies the signal, greatly improves the sensitivity, and has a wider range of applications.

The invention discloses an impurity-free Preyssler-type heteropoly acid and a preparation method thereof, belonging to the field of heteropoly acid preparation. The preparation method of the Preyssler type heteropolyacid comprises the following steps: adding phosphoric acid to the aqueous solution of sodium tungstate, and then reacting at 100-140° C. in the reactor for 12-24 hours, and the molar ratio of phosphoric acid to sodium tungstate is 4- 10:1; after cooling to room temperature, add ammonium chloride for suction filtration; wash with methanol solution and recrystallize; add a small amount of sorbic acid and ion-exchange the heteropolyacid solution; finally, vacuum dry the eluent. The preparation method of the impurity-free Preyssler-type heteropolyacid does not add any potassium ions, and at the same time saves the potassium acetate washing step, avoids the introduction of potassium ions into the product, and improves the yield and purity of the finished heteropolyacid, In addition, the preparation method has the advantages of simple process and convenient operation, and is suitable for industrialized large-scale production.

The invention discloses a method for extracting and analyzing the total DNA of microorganisms in ship's ballast water; according to the characteristics of ship's ballast water, the present invention uses two-stage filtration and freeze-fixation of 3 μm and 0.22 μm microporous membranes to strengthen the For the attachment of microorganisms on the filter membrane, the purity of the total DNA of microorganisms obtained by treating with cetyltrimethylammonium bromide (CTAB) meets the needs of direct subsequent molecular biology research, and the extracted DNA fragments can be amplified for 16SrDNA And denaturing gradient gel electrophoresis (DGGE). After digital analysis of the electrophoresis graph, the diversity of microbial populations in the ship’s ballast water can be judged. The specific bands are cut and recovered by gel cutting, cloned and sequenced, and BLAST sequence comparison is performed to obtain the ballast water. The types of water-dominant bacterial flora can be determined to determine the structure and composition of microbial communities, which will lay a foundation for future research on microbial diversity and ecological functions in ship ballast water and sediments.

The invention relates to a ready-to-resuscitate cryoprotectant for erythrocytes and a use method thereof, and relates to the field of cell technology. The composition of the cryoprotectant for erythrocytes provided by the invention includes 0.25-0.3mol / L trehalose, 5-7% (v / v ) of dimethyl sulfoxide, 1~3% (v / v) of human serum albumin. The erythrocyte cryoprotectant provided by the invention has simple components, and it is not necessary to strictly control the mixing speed between the erythrocytes and the erythrocyte cryoprotectant when the cells are frozen, just mix them directly, and the cryopreservation method is simple and time-consuming. The cryopreserved red blood cells of the present invention can be used without washing after resuscitation, which saves the tedious and time-consuming washing steps in the glycerol cryopreservation method and meets the needs of emergency blood users.

The invention relates to a method for preparing a white carbon black / solution-polymerized styrene-butadiene rubber nano composite material. In the invention, nano white carbon black powder and silanecoupling agent are fully mixed and condensation reaction is carried out at high temperature, organic modified nano white carbon black powder is obtained and added into solution-polymerized styrene-butadiene; the mixture of the white carbon black powder and solution-polymerized styrene-butadiene is stirred and removes solvent and then dried, thus obtaining the silicon dioxide / solution-polymerized styrene-butadiene rubber nano composite material prepared by co-polymerization. The invention expands the range of methods for preparing the nano composite material with solution composition. The adopted method using the organically modified white carbon black is simple and easy for operation and the solution composite technique is fast and convenient with low cost. The prepared co-polymerized rubber can be taken as master batch and is added with the white carbon black or added with other fillings for preparing novel nano composite material. The physical and mechanical properties and dynamic mechanical property of the rubber prepared by the method are better than the properties of coordinative amount of filling prepared by mechanical mixing method and the composition speed of subsequent fillings of the rubber prepared by the method is faster than that prepared by the mechanical method.

The invention discloses a regeneration method for iron oxide desulfurizer. The regeneration method comprises the following steps: (1) conducing oxidation treatment on the iron oxide desulfurizer through which sulphur penetrates, and mixing the oxidized iron oxide desulfurizer with catalyzed and cracked diesel oil at 40 to 150 DEG C for 0.1 to 10 hours, preferably, 1 to 3 hours; (2) conducting solid-liquid separation on the mixture obtained in step (1) to obtain a doctor solution and a desulfurizer material, cooling the liquid-phase to be 0 to 30 DEG C, preferably, 10 to 25 DEG C, recycling elemental sulfur, and drying the desulfurizer material to obtain a regenerative desulfurizer, wherein the doctor solution can be recycled. The regeneration method has the advantages that the sulfur capacity of the regenerative desulfurizer is high; the purity of recycled brimstone is relatively high; the operation is simple; the cost is low.

The invention discloses a coal tar hydrogenation catalyst and a preparation method thereof. The preparation method comprises the following contents: firstly preparing high-silicon pseudo-boehmite, and then mixing the obtained high-silicon pseudo-boehmite with an auxiliary agent The carrier is obtained after molding, drying and calcining, and finally the active metal components are introduced into the obtained carrier, and the catalyst is obtained after further drying and calcining. In the preparation method of the coal tar hydrogenation catalyst of the present invention, high-silicon pseudo-boehmite is used as a raw material in the preparation process, and an auxiliary agent is added in the preparation of the carrier at the same time, and enters into the alumina structure to form a water-resistant component, and then Improve the water resistance of the catalyst, and prepare a coal tar hydrogenation catalyst with good wear resistance and hydrogenation performance.

The present invention relates to a method for preparing ascorbic acid aliphatic ester, and said method includes the following steps: preparing a mixture containing 95-100 wt% of sulfuric acid, ascorbic acid and aliphatic acid, making the described mixture produce reaction to obtain the reaction produce mixture containing ascorbic acid aliphatic ester, and separating out ascorbic acid aliphatic ester from it, the mole ratio of ascorbic acid and aliphatic acid is 1 to less than 1.25, and the weight ratio of sulfuric acid and ascorbic acid is at least 6. The reaction mixture is mixed with ice whose volume dose is 2-10 times that of sulfuric acid and / or water whose temp. is below 5 deg.C, then mixed with an aromatic hydrocarbon solvent, and said mixture is hented, and cooled to obtain an organic phase, said organic phase is filtered and dried so as to obtain a solid, i.e. the invented product.

The invention discloses a method for extracting total DNAs of soil microorganisms at high purity. CTAB (Cetyltrimethyl Ammonium Bromide), lysozyme, protease and SDS (Sodium Dodecyl Sulfonate) act together to perform lysis of cells and simultaneously freeze and thaw the cells repeatedly; therefore, an excellent cell lysis effect is achieved; the humic acid is removed by utilizing PVP (Polyvinyl Pyrrolidone), CaC12 and BSA (Bull Serum Albumin); and the DNAs are precipitated by utilizing isopropanol so that the purity of the obtained DNAs is high. The method is simple and convenient; and without being purified, the DNAs obtained by the method can be used for subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (Denaturing Gradient Gel Electrophoresis) analysis. The purity of the extracted DNAs meets the requirement for directly performing subsequent molecular biological research; the extracted DNA segments can be subjected to 16SrDNA amplification and DGGE atlas analysis; glue cutting recovery and basic group sequencing can be performed on specific stripes of the DGGE band; unknown bacteria can be identified through comparison with the conventional sequences in an RDP (Ribosomal Database Project) database or by establishing a new sequence probe; and therefore, the structural composition of the microbial community can be determined, and a foundation can be laid for researching the structural diversity and ecological functions of the soil microorganisms in the rhizosphere of the mulberry in future.

The invention discloses a compound of high-whiteness clay and a preparation method thereof. The preparation method is as follows: firstly adding titanium tetrachloride solution into clay slurry to dissolve out the developing elements in the clay in the form of ions; secondly, adding phosphoric acid water solution to load the phosphate precipitation and titanium phosphate precipitation of the developing elements on the surface of the clay; and finally adding silicate solution or aluminate solution to ensure generated silica or alumina to compound the clay once again to obtain the high-whiteness clay. The invention has low cost, simple operation and less environmental pollution.

The invention discloses a synthesis method of an HZSM-5 molecular sieve adopting a dual template agent. The synthesis method comprises the steps that a silicon source, an aluminum source, a template agent, seed crystal and water are mixed in proportion and uniformly stirred to form gel; the gel is dried and ground into powder; the powder is crystallized in a reaction kettle containing water at the bottom; and a crystallized product is dried and calcined to obtain the product. The method does not use a raw material containing sodium ions, saves traditional ammonium exchange, washing and calcination processes, avoids separation of the product and mother liquid in the traditional crystallization technology, does not use expensive organic amine as the template agent, and has the characteristics of low cost, small waste liquid quantity, high yield and the like.