31results about How to "Eliminates washing steps" patented technology

The invention discloses a preparation method for an SCR catalyst for purifying oxynitride in motor vehicle exhaust .The preparation method includes the steps of preparation of an SSZ-13 molecular sieve, preparation of a Cu-SSZ-13 catalyst and preparation of an integrated SCR catalyst .The preparation method is simple, production cost is lowered, SSZ-13 crystallization time is short, crystallization is relatively pure, HN3-SCR catalytic activity is high, and the purification rate of oxynitride in motor vehicle exhaust is high.

The invention discloses a method for extracting total DNAs of soil microorganisms at high purity. CTAB (Cetyltrimethyl Ammonium Bromide), lysozyme, protease and SDS (Sodium Dodecyl Sulfonate) act together to perform lysis of cells and simultaneously freeze and thaw the cells repeatedly; therefore, an excellent cell lysis effect is achieved; the humic acid is removed by utilizing PVP (Polyvinyl Pyrrolidone), CaC12 and BSA (Bull Serum Albumin); and the DNAs are precipitated by utilizing isopropanol so that the purity of the obtained DNAs is high. The method is simple and convenient; and without being purified, the DNAs obtained by the method can be used for subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (Denaturing Gradient Gel Electrophoresis) analysis. The purity of the extracted DNAs meets the requirement for directly performing subsequent molecular biological research; the extracted DNA segments can be subjected to 16SrDNA amplification and DGGE atlas analysis; glue cutting recovery and basic group sequencing can be performed on specific stripes of the DGGE band; unknown bacteria can be identified through comparison with the conventional sequences in an RDP (Ribosomal Database Project) database or by establishing a new sequence probe; and therefore, the structural composition of the microbial community can be determined, and a foundation can be laid for researching the structural diversity and ecological functions of the soil microorganisms in the rhizosphere of the mulberry in future.

The invention discloses a method for extracting total DNA (deoxyribonucleic acid) of mulberry rhizosphere soil microorganisms by adopting a plurality of measures, which can achieve a good cell lysis effect by utilizing PVP (polyvinyl pyrrolidone) prewashing to remove humic acid, the synergy of CTAB (cetyltrimethyl ammonium bromide), lysozyme, protease and SDS (sodium dodecyl sulfate) to lyse cells and repetitively freezing and thawing the cells at the same time and utilizes PEG8000 to precipitate DNA, so that the purity of the obtained DNA is high. The adopted method is simple and convenient, and the DNA can be used in subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (denaturing gradient gel electrophoresis) analysis without needing to be purified. The extracted DNA is so pure as to meet the requirement of direct subsequent molecular biology research, an extracted DNA fragment can be used in the amplification and DGGE map analysis of 16SrDNA, moreover, the specific band gel extraction and base sequencing of a DGGE band can be carried out, comparison with the sequence existing in an RDP (ribosomal database project) database can be carried out, or a new sequence probe can be established to identify unknown bacteria, so that the composition of a microbial community structure can be determined, and thereby laying a foundation for the future research of the community structure diversity and the ecological functions of the mulberry rhizosphere soil microorganisms.

The invention discloses a method for extracting total DNAs (Deoxyribonucleic Acid) of soil microorganisms through PVP (Polyvinyl Pyrrolidone) pretreatment. Humic acid is removed through PVP pre-washing; CTAB (Cetyltrimethyl Ammonium Bromide), lysozyme, protease and SDS (Sodium Dodecyl Sulfonate) act together to perform lysis of cells and simultaneously freeze and thaw the cells repeatedly; therefore, an excellent cell lysis effect is achieved; and the DNAs are precipitated by utilizing isopropanol so that the purity of the obtained DNAs is high. The method is simple and convenient; and without being purified, the DNAs obtained by the method can be used for subsequent PCR (Polymerase Chain Reaction) analysis and DGGE (Denaturing Gradient Gel Electrophoresis) analysis. The purity of the extracted DNAs meets the requirement on directly performing subsequent molecular biological research; the extracted DNA segments can be subjected to 16SrDNA amplification and DGGE atlas analysis; glue cutting recovery and basic group sequencing can be performed on specific stripes of the DGGE band; and unknown bacteria can be identified through comparison with the conventional sequences in an RDP (Ribosomal Database Project) database or by establishing a new sequence probe; and therefore, the structural composition of the microbial community can be determined, a foundation can be laid for researching the structural diversity and ecological functions of the soil microorganisms in the rhizosphere of the mulberry in future.

The invention discloses a preparation method of a lithium ion battery positive electrode composite material and a precursor thereof, and in particular relates to a novel method for preparing a high-purity low-cost binary or ternary precursor and preparing a high-performance lithium ion battery binary or ternary positive electrode composite material by utilizing the precursor, belonging to the technical field of new energy materials and the preparation thereof. The method comprises the following specific steps: (1) putting salt-type solid raw materials of two or three of nickel, cobalt and manganese with crystal water into a reactor, and heating the materials to the molten state; (2) introducing ammonia under the protection of inert gas, supplementing a small amount of water or no water according to the solubility of salts at different temperatures, stirring and reacting; (3) evaporating ammonium salt after full reaction, taking the solid out, and drying so as to get the amorphous binary or ternary positive electrode composite material precursor; and (4) mixing the precursor with lithium carbonate according to a certain ratio, and further preparing the lithium ion battery positive electrode composite material through a two-section sintering method. The precursor synthetic method is simple, sodium hydroxide is avoided from being used, separation and purification are not required, and the high-purity positive electrode composite material precursor which has no impurities basically can be obtained; furthermore, no industrial waste water is discharged, and the by-product ammonium salt can further generate economic benefit; and the positive electrode composite material prepared by the precursor has excellent performances, and is convenient for industrialization.

A process for producing thermally inhibited starch, specifically thermally inhibited non- pregelatinized granular starch, is described, resulting in a viscostable starch product. The process comprising providing an alkaline starch, specifically an alkaline non-pregelatinized granular starch,having a pH of at least 8; subjecting the starch to a hydrothermal treatment, specifically to obtain a hydrothermally treated non-pregelatinized granular starch, said hydrothermal treatment being at a temperature of 45-200DC with steam at a steam pressure of 0.1-15 bar or a gas mixture comprising water vapor at a partial water vapor pressure of 0.1-1 bar; dehydrating the starch, specifically the hydrothermally treated non-pregelatinized granular starch,to a moisture content of 2 wt% or lower and subjecting the starch to a thermal treatment by heating the starch to a temperature of 120-190DC to obtain viscostability,cooling and optionally further processing the starch.

The invention discloses a fluidized bed hydrogenation catalyst and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an alumina carrier; (2) mixing the alumina carrier with a first active metal solution, and drying to obtain a catalyst precursor A; and (3) introducing a second active metal component to the catalyst precursor A, and drying and roasting to obtain the catalyst. According to the preparation method of the fluidized bed hydrogenation catalyst, active metal is added in the preparation of the carrier for effective adsorption and occupation, so that the dispersion of the metal is further optimized, and the fluidized bed hydrogenation catalyst which is high in unit surface acid content, wear-resistant and good in hydrodesulfurization performance is prepared.

The invention relates to an accurate immune sensing method with an adjustable linear range and a portable biological resistance sensing measurement device.The detection device comprises a detection pipeline, one end of the detection pipeline is connected with a vacuum pump through threads, a liquid suction head is arranged at the other end of the detection pipeline, a micro-channel is formed in the detection pipeline, and a battery is arranged on the side face of the detection pipeline; the battery provides power for the concentration measurement module, two detection electrodes are arranged in the measurement module and connected to the two sides of the micro-channel respectively, a kit is arranged in the joint of the detection pipeline and the gun head, and a vibration device is arranged on the detection pipeline on the outer side of the kit. According to the present invention, the detection limit linear range is adjustable, the sensitivity is high, the stability is good, the detection cost is low, and the detection speed is fast; high pressure generated by the vacuum pump is matched with filtration of the microporous filter membrane, so that the problems of blockage of large-particle non-to-be-detected substances in the microchannel and adsorption, aggregation and blockage of to-be-detected substances in the microchannel can be well solved.

The invention discloses a method for preparing a positive composite positive material precursor for lithium ion batteries, and the method is simple and convenient in technology. The method comprises the following specific steps: (1) putting any two or three salt solid raw materials of nickel, cobalt and manganese with crystal water into a reactor, and heating to a molten state; (2) introducing ammonia gas under inert gas shielding, carrying out pressurization until 1.2 to 1.4 Mpa, appropriately supplementing a small mount of water or not adding water according to the solubility of the salt atdifferent temperatures, and reacting while stirring; (3) evaporating ammonium salt after the reaction is completed, taking out a solid, and drying to obtain an amorphous binary or ternary positive composite material precursor; and (4) mixing the precursor with lithium carbonate in proportion, and carrying out a two-stage sintering process to prepare the positive composite material for lithium ionbatteries. The positive composite material prepared by the precursor is excellent in performance, and is convenient for industrialization.

The invention discloses impurity-free Preyssler type heteropolyacid and a preparation method thereof, and belongs to the field of heteropolyacid preparation. The preparation method of the impurity-free Preyssler type heteropolyacid comprises the following steps: adding phosphoric acid into an aqueous solution of sodium tungstate, and then reacting for 12-24 hours at 100-140 DEG C in a reaction kettle, wherein the molar ratio of phosphoric acid to sodium tungstate is (4-10): 1; after cooling to room temperature, adding sodium chloride for suction filtration; washing with a methanol solution andrecrystallizing; adding a small amount of sorbic acid and carrying out ion exchange on the heteropolyacid solution; and finally, carrying out vacuum drying on the eluent. According to the impurity-free Preyssler type heteropolyacid preparation method, no potassium ions are added, meanwhile, the potassium acetate washing step is omitted, introduction of the potassium ions into the product is avoided, the yield and purity of the heteropolyacid finished product are improved, and the preparation method is simple in process, convenient to operate and suitable for industrial large-scale productionand use.

The invention discloses a preparation method of a mounting medium capable of simultaneously marking a cell nucleus. The preparation method comprises the following steps: 1) preparing 1 mol/L of TrizmaBase; 2) preparing 1 mol/L of NaH2PO4.2H2O; 3) preparing a Tris-phosphate buffer; 4) preparing glycerinum with the concentration of 50%; and 5) preparing the mounting medium. According to the prepared mounting medium capable of simultaneously marking the cell nucleus, the steps of dyeing and washing are omitted, the experiment flow is effectively simplified, the time cost is reduced, and the working efficiency is improved. The mounting medium can be used to mark and mount the cell nucleuses of samples like a frozen section, a paraffin section, a cell coverslip and a blood smear. The mountingmedium not only can be used to observe through a fluorescence microscope, but also can be used to observe through a laser scanning confocal microscope.

The invention discloses a coal tar hydrogenation catalyst and a preparation method thereof. The preparation method comprises the following steps: firstly preparing high-silicon pseudo-boehmite, then mixing the obtained high-silicon pseudo-boehmite with an auxiliary agent, molding, drying and roasting to obtain a carrier, finally introducing an active metal component onto the obtained carrier, and further drying and roasting to obtain the catalyst. According to the preparation method of the coal tar hydrogenation catalyst, the high-silicon pseudo-boehmite is adopted as a raw material in the preparation process, and the auxiliary agent is added in the carrier preparation process and enters an aluminum oxide structure to form a water-resistant component, so that the water resistance of the catalyst is improved, and the coal tar hydrogenation catalyst with good wear resistance and hydrogenation performance is prepared.

The invention relates to a preparation method with a simple process for a lithium ion battery positive electrode composite material. The preparation method comprises the following steps of (1) placingarbitrary two or three of salt solid raw materials of nickel, cobalt and manganese with crystallization water in a reactor, and performing heating to a melting state; (2) introducing ammonia gas under protection of inert gas, pressurizing to 1.2-1.4MPa, appropriately supplementing a few amount of water or not adding water according to solubility of the salts under different temperatures, and performing stirring and reaction; (3) evaporating an ammonium salt after reaction is completed, taking out a solid, and drying the solid to obtain an amorphous binary or ternary positive electrode composite material precursor; and (4) mixing the precursor and lithium carbonate according to a certain proportion, wherein the lithium ion battery positive electrode composite material can be prepared by atwo-segment sintering method. The positive electrode composite material prepared from the precursor is excellent in performance, and industrialization is facilitated.