Precise immunosensing method with adjustable linear range and portable biological resistance sensing measurement device

A sensing measurement, portable technology, applied in the fields of biosensing and food safety, can solve the problems that the signal is easily interfered by external complex factors, reduce the detection efficiency, and the volume of the instrument is large, so as to achieve the sensitivity of the method, good suspension, and The effect of low detection cost

Pending Publication Date: 2022-07-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The invention patent is based on the principle of electrochemical biosensing, but the resolution of this method is low, and it takes an order of magnitude difference between the sample concentrations to detect obvious signal changes, resulting in low accuracy in actual sample detection using this method
In addition, a multi-channel particle detection device and detection method for detecting micron particles (publication number CN111413264A) discloses that a plurality of small hole test tubes are arranged on the box, and small holes are opened on the small hole test tubes. There are positive and negative electrodes on the outside, and the lifting mechanism is used to control the lifting of the small hole test tube. The process of the target object in the sample pool entering the small hole will cause the resistance signal between the first electrode and the second electrode to change. After signal conversion Converting the resistance signal into a digital signal output, this invention can realize the simultaneous detection of multiple samples, the detection efficiency is high and the detection cost can be reduced, but the cost of the device is high, the instrument is large in size, inconvenient to carry, and the small hole needs to be cleaned every time And the electrode reduces the detection efficiency. The signal collected by the electrode needs to be multi-amplified and filtered by the signal processing system to obtain an obvious voltage pulse signal. The signal is easily interfered by external complex factors.

Method used

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  • Precise immunosensing method with adjustable linear range and portable biological resistance sensing measurement device
  • Precise immunosensing method with adjustable linear range and portable biological resistance sensing measurement device
  • Precise immunosensing method with adjustable linear range and portable biological resistance sensing measurement device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 Construction of a portable bio-resistance sensing measurement device

[0079] The present invention provides a portable bioresistance sensing and measuring device.

[0080] see Figure 1-3 As shown in the figure, a portable bio-resistance sensing measurement device, the detection device includes a detection pipe 1, one end of the detection pipe 1 is connected to a vacuum pump 2 by a thread, the other end of the detection pipe 1 is provided with a liquid suction head 3, and the interior of the detection pipe 1 is provided with a micro Channel 4, the side of the detection pipe 1 is provided with a battery 5. The battery 5 provides power to the concentration measurement module 6. There are two detection electrodes 7 in the measurement module 6. The detection electrodes 7 are respectively connected to both sides of the microchannel 4. A reagent box 8 is arranged inside the connection of the liquid head 3 , and a vibration device 9 is arranged on the detection pip...

Embodiment 2

[0089] Example 2 Exploration on the correlation between the conductivity of the microchannel and the applied current, the length of the microchannel, the inner diameter of the microchannel and the flow rate

[0090] Using the device of Example 1, the following experiments were carried out:

[0091] Experiment 1): Pour PS microspheres (1μm, 3μm) solutions of different diameters into the microchannel, detect the voltage difference of the closed-loop circuit under different currents (0, 100, 200, 300, 400μA), and investigate the PS microspheres The relationship between particle size and capillary conductivity, the results are as follows Image 6 shown.

[0092] Test 2): Pour PS microspheres (1 μm, 3 μm) solutions of different diameters into the microchannel, and detect closed loops at different flow rates (50 μL / min, 100 μL / min, 150 μL / min, 200 μL / min, 250 μL / min) The voltage difference of the circuit is investigated to investigate the relationship between the particle size of ...

Embodiment 3

[0096] Example 3 Modification of magnetic beads, PS microspheres and click reagents with biological recognition molecules

[0097] 1. Activation of magnetic beads

[0098] (1) Take 2 mg of magnetic beads (average diameter 1 μm) into a centrifuge tube, wash twice with 500 μL MEST (10 mM MES, 0.05% Tween20, pH 6.0), and remove the supernatant by magnetic separation; (2) Use 10 mM MES ( pH 6.0) to prepare 5 mg / mL EDC solution and 5 mg / mL NHS solution; (3) add 100 μL EDC (5 mg / mL) and 50 μL NHS (5 mg / mL) to the centrifuge tube containing magnetic beads, respectively, and use a vortexer Mix well to fully suspend the magnetic beads, dilute to 500 μL with MES, place on a rotary mixer, activate at 37°C for 30 min; (4) Magnetic separation, remove the supernatant, wash twice with 500 μL MEST, magnetically separate, and remove the upper layer clear liquid. After the above steps, the carboxyl groups on the surface of the magnetic beads have been activated.

[0099] 2. Activation of PS ...

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Abstract

The invention relates to an accurate immune sensing method with an adjustable linear range and a portable biological resistance sensing measurement device.The detection device comprises a detection pipeline, one end of the detection pipeline is connected with a vacuum pump through threads, a liquid suction head is arranged at the other end of the detection pipeline, a micro-channel is formed in the detection pipeline, and a battery is arranged on the side face of the detection pipeline; the battery provides power for the concentration measurement module, two detection electrodes are arranged in the measurement module and connected to the two sides of the micro-channel respectively, a kit is arranged in the joint of the detection pipeline and the gun head, and a vibration device is arranged on the detection pipeline on the outer side of the kit. According to the present invention, the detection limit linear range is adjustable, the sensitivity is high, the stability is good, the detection cost is low, and the detection speed is fast; high pressure generated by the vacuum pump is matched with filtration of the microporous filter membrane, so that the problems of blockage of large-particle non-to-be-detected substances in the microchannel and adsorption, aggregation and blockage of to-be-detected substances in the microchannel can be well solved.

Description

technical field [0001] The invention belongs to the fields of food safety and biological sensing, and in particular relates to a precise immune sensing method with adjustable linear range and a portable biological resistance sensing measuring device. Background technique [0002] The biosensing system mainly includes biological recognition molecules, signal conversion devices, photoelectric signal amplification devices and other parts. Common biosensors can be divided into three categories: optical biosensors, electrochemical biosensors and piezoelectric immunobiosensors. Optical biosensors have the advantages of less sample consumption and fast response speed, but the instruments are large in size, complex in frequency signal processing, expensive and complicated in operation. Piezoelectric immunosensors have short analysis time and good stability, but this type of sensor needs to couple biological recognition molecules on the detection substrate. The coupling time is long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/06G01N27/00G01N27/327G01N33/543G01N33/558B01L3/00
CPCG01N27/06G01N27/00G01N27/3275G01N33/54326G01N33/558B01L3/5027
Inventor 陈翊平鲁鹏冯牛周翠云周阳刘谱悦胡筱波
Owner HUAZHONG AGRI UNIV
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