Preparation method of mounting medium capable of simultaneously marking cell nucleus

A technology of mounting agent and cell nucleus, which is applied in the field of biomedicine, can solve the problems of time-consuming and cumbersome steps, and achieve the effects of saving time and cost, simplifying the experimental process, and improving work efficiency

Inactive Publication Date: 2018-04-13
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Each fluorescent label requires multiple washes, which is cumbersome and time-consuming

Method used

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the examples, but the present invention is not limited to the following examples, it can be foreseen that those skilled in the art may produce various changes in the implementation under the condition of combining the prior art.

[0020] A preparation method for a mounting agent capable of simultaneously labeling cell nuclei, comprising the steps of:

[0021] 1). Prepare 1mol / L Trizma Base: Dissolve 12.11g Trizma Base in 100ml deionized water and set aside;

[0022] 2). Prepare 1mol / L NaH 2 PO 4 2H 2 O: Take 15.62g NaH 2 PO 4 2H 2 O was dissolved in 100ml deionized water and set aside;

[0023] 3). Preparation of Tris-phosphate buffer: take 50ml of 1mol / L Trizma Base, add 1mol / L NaH 2 PO 4 2H 2 O adjusts pH to 7.6, for subsequent use;

[0024] 4). Prepare 50% glycerin: take 100ml glycerin, add 100ml deionized water, mix well, autoclave, and set aside;

[0025] 5). Preparation of mountin...

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Abstract

The invention discloses a preparation method of a mounting medium capable of simultaneously marking a cell nucleus. The preparation method comprises the following steps: 1) preparing 1 mol / L of TrizmaBase; 2) preparing 1 mol / L of NaH2PO4.2H2O; 3) preparing a Tris-phosphate buffer; 4) preparing glycerinum with the concentration of 50%; and 5) preparing the mounting medium. According to the prepared mounting medium capable of simultaneously marking the cell nucleus, the steps of dyeing and washing are omitted, the experiment flow is effectively simplified, the time cost is reduced, and the working efficiency is improved. The mounting medium can be used to mark and mount the cell nucleuses of samples like a frozen section, a paraffin section, a cell coverslip and a blood smear. The mountingmedium not only can be used to observe through a fluorescence microscope, but also can be used to observe through a laser scanning confocal microscope.

Description

technical field [0001] The invention relates to biomedicine, in particular to a method for preparing a mounting agent capable of simultaneously marking cell nuclei. Background technique [0002] In biomedical experiments, it is often necessary to stain specific components in cells and tissues with fluorescent dyes, and then observe them under a fluorescent microscope. Also, fluorescent markers stain the nuclei in almost all samples. The currently commonly used experimental method is to sequentially label the specific components and nuclei in the cells, seal the slides with a mounting medium, and then observe them under a fluorescent microscope. Each fluorescent label requires multiple washes, which is cumbersome and time-consuming. Since the fluorescence is easily quenched, the shorter the operation time of the fluorescent labeling, the better, so it is necessary to find a way to shorten the fluorescent staining. Contents of the invention [0003] The purpose of the pre...

Claims

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Application Information

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IPC IPC(8): G01N1/36G01N21/64
CPCG01N1/36G01N21/64G01N21/6402
Inventor 杨明理何波
Owner ZUNYI MEDICAL UNIVERSITY
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