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Application of a kind of brefeldin a in the preparation of inflammatory factor activity inhibitor medicine

A Brefeldin bacteria and activity inhibitor technology, applied in the application field of Brefeldin A in the preparation of inflammatory factor activity inhibitor drugs, to achieve the effect of improving pathological changes

Active Publication Date: 2020-12-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, Brefeldin A, as a protein transport inhibitor, has become an important molecular tool and has been widely used in the study of signal transduction pathways in mammals, but whether it is effective for acute lung injury and other inflammations has not yet been studied

Method used

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  • Application of a kind of brefeldin a in the preparation of inflammatory factor activity inhibitor medicine
  • Application of a kind of brefeldin a in the preparation of inflammatory factor activity inhibitor medicine
  • Application of a kind of brefeldin a in the preparation of inflammatory factor activity inhibitor medicine

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Embodiment 1

[0030] 1 Materials and methods

[0031] 1.1 Experimental animals C57 / BL6 mice, male, body weight 19-21g, clean grade, provided by the Experimental Center of Zhejiang University School of Medicine, certificate number: (SCXK2013-0016). All operations and experimental procedures complied with the "Regulations on the Management of Experimental Animals". Experimental environment: constant temperature (22±2)°C, humidity 60%-70%, free drinking and eating.

[0032] 1.2 Reagents and instruments LPS (Escherichia coli LPS O55:B5, L2880, sigma); Brefeldin A (BFA, sigma); dexamethasone (DXM, 5mg / ml, Zhejiang Xianju Pharmaceutical Co., Ltd.), myeloperoxidase (myeloperoxidase, MPO) kit (Nanjing Jiancheng Biotechnology Co., Ltd., A044); TNF-α (tumornecrosis factor-α), IL-1β (interleukin-1β) and IL-6 (interleukin-6) ELISA kits were purchased from R&D Systems (R&D Systems, Mineapolis, MN, USA, DY410, DY401, DY406); Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, 500-0120); Cyclic Adeno...

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Abstract

The invention discloses application of brefeldin A in preparation of an inflammatory factor active inhibitor medicine. It is discovered that the brefeldin A can significantly reduce release of TNF-alpha in MH-S and generation of KC in an MLE-12 cell, significantly improve the pathological change of the lung tissue of a mouse, reduce the content of leucocytes and TNF-alpha in BALF, significantly reduce the activity of MPO in the lung tissue of the mouse, significantly improve the cAMP level and significantly inhibit phosphorylation of ERK. The Brefeldin A can achieve a protection effect on acute lung injuries, and the mechanism of the Brefeldin A may be related to ways of increasing the content of cAMP in cells, inhibiting the phosphorylation of the ERK and the like. According to the application, a new research direction is provided for the therapeutic target of the acute lung injuries, by adjusting protein translocation in the cells, generation and secretion of the inflammatory factormay be inhibited, and therefore development of inflammation is hindered.

Description

[0001] (1) Technical field [0002] The present invention relates to the application of brefeldin A, in particular to the application of brefeldin A in the preparation of inflammatory factor activity inhibitor drugs. [0003] (2) Background technology [0004] Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are acute and progressive hypoxic respiratory failure caused by various pathogenic factors in and outside the lungs other than cardiogenic. The specific molecular mechanisms of ALI / ARDS disease progression are poorly understood, and there are no specific therapeutic drugs for ALI / ARDS. Lipopolysaccharide (LPS) is the main component of Gram-negative bacteria and is widely used to induce ALI / ARDS to study the molecular mechanism of acute inflammatory injury in the lung. Brefeldin A (BFA) is a macrolide antibiotic synthesized by fungi. Separated from Penicillium decumben fermentation broth, it can induce the decomposition of Golgi apparatus, anti-compet...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P11/00
CPCA61K31/365
Inventor 汤慧芳
Owner ZHEJIANG UNIV
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