7results about How to "Improve pathological changes" patented technology

This invention relates to the application of mitochondrial formulations in the preparation of drugs for treating chronic obstructive pulmonary disease (COPD). The mitochondrial formulations contain active mitochondria isolated from healthy donor cells, with a particle size of 400–1000 nm, possessing normal mitochondrial membrane potential and ATP generation function, and capable of being internalized by mammalian COPD lung epithelial cells. In vitro cell experiments have confirmed that this mitochondrial formulation can repair CSE-induced mitochondrial dysfunction in lung epithelial cells, inhibit mPTP opening, reduce mtDNA leakage into the cytoplasm, thereby blocking the activation of the cGAS-STING signaling pathway and reducing the release of inflammatory factors such as IL-1β and TNF-α. In vivo experiments in COPD mouse models show that tail vein injection of the mitochondrial formulation can effectively accumulate in lung tissue, significantly improving lung pathological changes such as emphysema and collagen fiber deposition in model mice. This invention provides a novel strategy for COPD treatment based on repairing mitochondrial function and intervening in inflammatory signaling pathways.

This invention belongs to the field of traditional Chinese medicine preparations, and relates to the preparation method and application of a compound preparation of Yao Yu effervescent tablets with anti-rheumatoid arthritis effect. The compound preparation comprises the following traditional Chinese medicine components: 4 parts by weight of Dioscorea cirrhosa, 3 parts by weight of Tinleaf Vine, 2 parts by weight of Camphor Tree, 2 parts by weight of Rhus chinensis, 3 parts by weight of Rhododendron molle, 1 part by weight of Ephedra sinica, 2 parts by weight of Semi-Acer palmatum, 2 parts by weight of Gynostemma pentaphyllum, and 1 part by weight of Cinnamomum cassia. The preparation steps include: placing Dioscorea cirrhosa, Tinleaf Vine, Camphor Tree, Rhus chinensis, Rhododendron molle, Gynostemma pentaphyllum, Semi-Acer palmatum, Gynostemma pentaphyllum, and Cinnamomum cassia in 6000 parts by weight of 70% ethanol. Extract for 2 hours, repeat three times, filter, and combine the filtrates; concentrate, freeze-dry, pulverize, and pass through an 80-mesh sieve for later use; mix 27.5% freeze-dried powder with 26.45% citric acid, 34.11% sodium bicarbonate, 5.61% PEG6000, and 6.33% lactose evenly, pass through an 80-mesh sieve, and prepare a soft mass using anhydrous ethanol as a wetting agent; pass the soft mass through a 20-mesh sieve and dry at 37℃ for 30 minutes to obtain compound preparation granules; granulate through a 20-mesh sieve for granulation, add lubricant PEG6000 and mix evenly; compress into tablets to obtain compound preparation effervescent tablets. This invention, through pharmacological experiments, confirms that the Yaoyu effervescent tablet compound preparation has a good anti-rheumatoid arthritis effect: it can reduce local inflammatory mediators and serum IL-1β, IL-6, TNF-α, and PEG2 levels, downregulate MDA and increase SOD to improve oxidative stress, inhibit the expression of MMP3, COX-2, and P38 proteins in the synovial membrane, and reduce pathological tissue damage. The medium and high dose groups showed the best effect, and it has a good therapeutic and protective effect on the ankle joint of AA rats, with good clinical application value.

The invention belongs to the technical field of traditional Chinese medicine, and relates to application of a yin-nourishing and lung-clearing composition in preparation of medicine for preventing and / or treating pulmonary fibrosis, and the yin-nourishing and lung-clearing composition is prepared from the following raw materials: rehmannia, radix scrophulariae, radix ophiopogonis, moutan bark, bulbus fritillariae cirrhosae, radix paeoniae alba, mint and liquorice. The invention provides the application of the yin-nourishing and lung-clearing composition in preparing the medicine for preventing and / or treating the pulmonary fibrosis, bleomycin is injected into the trachea to construct a pulmonary fibrosis mouse model, and the effect of the yin-nourishing and lung-clearing composition on treating the pulmonary fibrosis is determined from the three aspects of the lung coefficient, the pathological change and the hydroxyproline content of a model animal. Basic data support is provided for increasing clinical application of the composition for nourishing yin and clearing away lung-heat.

The invention discloses a screening method of a traditional Chinese medicine monomer composition for resisting heart failure. The method comprises the following steps: 1, carrying out water extraction and enrichment on astragalus membranaceus and monkshood to obtain astragalus membranaceus and monkshood soup enriched components; 2, synthesizing myocardial mitochondrial membrane coated magnetic beads and myocardial lysosome membrane coated magnetic beads; 3, gathering enriched components of the astragalus mongholicus-monkshood decoction, and carrying out ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry to obtain components of songorine, neoline, calycosin and formononetin as traditional Chinese medicine monomer composition; the traditional Chinese medicine monomer composition is prepared by dissolving songlin, neoline, calycosin and formononetin in a sodium carboxymethyl cellulose solution. The synthesized myocardial mitochondrial membrane coated magnetic beads and the myocardial lysosome membrane coated magnetic beads are used for adsorbing and identifying active ingredients in the astragalus mongholicus-monkshood decoction, the effective ingredient composition for resisting heart failure is successfully screened, and the preparation method is simple and easy to implement; the traditional Chinese medicine monomer composition plays a role in preparing anti-heart-failure medicines by improving heart functions and the like.

The application relates to an application of a traditional Chinese medicine composition in a drug for chronic obstructive pulmonary disease, characterized in that the traditional Chinese medicine composition comprises the following components in parts: Magnolia officinalis 1-100 parts, Strychnos ignatii 1-100 parts, Toddalia asiatica 1-100 parts, Ephedra 1-100 parts, Amygdalus 1-100 parts, Notopterygium 1-100 parts, Zingiber officinale 1-100 parts, Pogostemonis 1-100 parts, Perilla 1-100 parts, Atractylodes 1-100 parts, Poria cocos 1-160 parts, Atractylodes macrocephala 1-120 parts, Gypsum fibrosum 1-100 parts, Fructus Crataegi 1-100 parts, Fructus Junci 1-100 parts, Fructus Hordei 1-100 parts, Lumbricus 1-100 parts, Radix et Rhizoma Veratri 1-100 parts, Herba Eupatorii Fortunei 1-100 parts, Semen Lepidii 1-100 parts and Semen Platycladi 1-100 parts.

The application discloses a Mongolian medicine characteristic enema for treating ulcerative colitis and relates to the technical field of biological medicine. The enema is prepared from a Mongolian medicine composition. The Mongolian medicine composition comprises the following components in parts by weight: Punica granatum 25-35 parts, Cinnamomum cassia 15-25 parts, cold water stone 15-25 parts, Amomum villosum 15-25 parts, Forsythia suspense 15-25 parts, Curcuma longa 15-25 parts, Terminalia chebula 15-25 parts, dried ginger 15-25 parts, Calvatia 15-25 parts, Caulis Sargentodoxae 15-25 parts, Radix Glycyrrhizae 5-15 parts, Platycladus orientalis 5-15 parts, Amomum kravanh 5-15 parts, alkali 5-15 parts and Polygonatum 5-15 parts. The Mongolian medicine characteristic enema has the advantages of improving the local effect of the medicine, reducing adverse reactions, having clear clinical curative effect and being innovative in the Mongolian medicine characteristics, and can provide a safe, effective and economical treatment scheme for UC patients.

The invention discloses an application of spermidine and pharmaceutically acceptable salts thereof in preparation of drugs for treating DKD, which can significantly reduce UACR, present a dose-dependent trend, improve renal function indexes, reduce blood sugar level and improve renal histopathologic change, thereby alleviating diabetic nephropathy related kidney injury and delaying disease progression.