Method for Tissue Culture of Aromatic Plant Lingling Xiangqing

A tissue culture and aromatic plant technology, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as no tissue culture research reports, and achieve the benefits of multiplication, high speed, and promotion of large-scale production. Effect

Active Publication Date: 2020-11-24
MINZU UNIVERSITY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] People's research on Cymbidium genus has focused on the extraction and analysis of phytochemical components such as Phytochrome japonica, Pearlescent Cymbidium, Milky White Cymbidium, and Cymbidium, but there are no research reports on tissue culture.

Method used

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  • Method for Tissue Culture of Aromatic Plant Lingling Xiangqing
  • Method for Tissue Culture of Aromatic Plant Lingling Xiangqing
  • Method for Tissue Culture of Aromatic Plant Lingling Xiangqing

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Experimental program
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Effect test

Embodiment

[0075] 1. Acquisition of materials

[0076] The material used in this experiment is Lingling Xiangqing seeds, collected from an alpine meadow at an altitude of 2100-2330m in Dongling Mountain, Beijing (40°1'51" north latitude, 115°27'19" east longitude).

[0077] 2. Disinfection treatment of seeds and cultivation of sterile seedlings

[0078] The best disinfection program is: alcohol disinfection for 30s, 10% hydrogen peroxide disinfection for 15 minutes, and the pollution rate is 0.

[0079] Inoculate the sterilized Lingling Xiangqing seeds in MS medium, and start to germinate in about 4 days; the germination rate reaches 98.0% in about 15 days, and each seed grows 2 cotyledons; when cultivated to 20 days, 1-2 true leaves are produced ( figure 1 ).

[0080] 3. Induction of callus

[0081] 3.1 Induction effect of 2,4-D

[0082] The leaves and petioles of the 30-day-cultivated seedlings of C. japonica were used as explants. The explants were respectively inoculated in MS ...

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Abstract

The invention provides a tissue cultivation method of an aromatic plant (anaphalis hancockii). The method includes the following step of a1, cultivating an adventitious bud of the anaphalis hancockiiin a rooting culture medium to obtain a regeneration seedling, wherein the rooting culture medium contains IAA with the concentration of 0.1-2.0 mg / L or IBA with the concentration of 0.1-2.0 mg / L or NAA with the concentration of 0.1-2.0 mg / L. By adding plant growth regulators of specific types and concentrations to culture mediums applied in all the stages of tissue cultivation of the anaphalis hancockii, the reproduction coefficient of the anaphalis hancockii is high, the speed is high, a large number of regeneration seedlings can be obtained within a short period of time, the propagation ofthe anaphalis hancockii is facilitated, and the large-scale production of the anaphalis hancockii is promoted.

Description

technical field [0001] The invention relates to a method for tissue culture of the aromatic plant Bollia chinensis. Background technique [0002] Anaphalis hancockii Maxim is a plant of the genus Anaphalis in the Compositae family. The whole plant has an aromatic smell and is one of the important spice plants. Lingling Xiangqing has high application value, and its whole plant can extract aromatic oil; it can also be used as an ornamental plant, a natural dried flower material, with a fragrance that lasts for several years; it can be used medicinally, the whole plant is used as medicine, and has heat-clearing properties Detoxification effect. In addition, it also has the effect of killing insects. [0003] People's research on Cymbidium genus has focused on the extraction and analysis of phytochemical components such as Phytocymum chrysalis, Pearlescent Cymbidium, Milky Chrysanthemum, and Cymbidium, but there are no research reports on tissue culture. Contents of the inve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王俊丽郝娜马伟伟黄航君李依杜嘉琳卢娇
Owner MINZU UNIVERSITY OF CHINA
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