A method for detecting the distribution of chloroplasts in rice leaves after light treatment

A light treatment and rice technology, applied in the field of plant light signal research, can solve the problem of difficult observation of rice leaves, and achieve the effect of clear observation results and simple operation process.

Active Publication Date: 2021-09-28
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting the distribution of chloroplasts in rice leaves after light treatment, so as to solve the problem that rice leaves are difficult to observe

Method used

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  • A method for detecting the distribution of chloroplasts in rice leaves after light treatment
  • A method for detecting the distribution of chloroplasts in rice leaves after light treatment
  • A method for detecting the distribution of chloroplasts in rice leaves after light treatment

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Embodiment 1

[0047] 1. Cultivation of rice seedlings

[0048] 1) Pick out the plump seeds from the harvested seeds, put them in a plate, add pure water, it is better to have no seeds, such as figure 1 A-B are shown.

[0049] 2) Place the plate at 30°C for 2-3 days to accelerate germination. Change the pure water every morning and evening, pay attention to the germination, if figure 1 C shown.

[0050] 3) When the seeds germinate and sow in the PCR plate, the PCR plate cuts off the bottom of each well before sowing. The sowing density should not be too high, and one seed is sown in 3 holes to prevent the growth from being affected by excessive close planting.

[0051] 4) After the sowing is completed, first cultivate in water for 3 days to prevent the flooding of green algae caused by over-nutrition, and then replace it with rice culture medium for cultivation, and change it once a week to ensure that the culture medium is sufficient every day, and continue to cultivate for 2 weeks. The ...

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Abstract

The invention discloses a method for detecting the distribution of chloroplasts in rice leaves after light treatment, and belongs to the technical field of plant light signal research. The method comprises: (1) taking the rice seeds to be tested, and obtaining rice seedlings by hydroponics; (2) placing the rice seedlings in a dark environment for 12-36h; The seedlings are placed under different light conditions for cultivation; (4) clipping the middle region of the flag leaf of the rice seedlings treated with light, placing them in a fixative solution, and fixing the leaves; (5) taking out the fixed leaves from the fixative solution, Make slices, and then use laser confocal microscope to observe and record the distribution of rice chloroplasts in cells. The operation process of the invention is simple, and the observation result is clear, real and intuitive. The method provided by the invention can be used to study the relationship between rice chloroplasts and light signals, and provide help for further analyzing the regulation mechanism of rice chloroplast light signals.

Description

technical field [0001] The invention relates to the technical field of plant light signal research, in particular to a method for detecting the distribution of chloroplasts in rice leaves after light treatment. Background technique [0002] As the site of plant photosynthesis, chloroplasts absorb carbon dioxide, release oxygen, and synthesize organic matter. With the gradual deepening of light signal research in various plants, the research and identification of chloroplasts to different light intensities and different light qualities is of great significance. [0003] Rice (Oryza Sativa) is one of the important food crops, and ensuring a high yield of rice is an issue related to the national economy and the people's livelihood. Rice is a monocotyledonous plant of Gramineae, which likes temperature and humidity. The indoor hydroponic method of rice seedlings is widely used in various aspects of rice research because of its rapidity and convenience. The key to ensuring its h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/04A01G31/00C12Q1/02G01N21/63
CPCA01G7/04A01G31/00G01N21/6486G01N33/4833Y02P60/14
Inventor 于彦春张燕莉武丽敏陈飞熊二辉徐俊锋
Owner HANGZHOU NORMAL UNIVERSITY
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