Mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as matrix and mass spectrum imaging method thereof

A technology of dihydroxybenzoic acid and mass spectrometry imaging, applied in the field of mass spectrometry imaging, can solve the problems of poor phthalate mass spectrometry imaging effect, inability to accurately describe the spatial distribution, etc., to enhance ionization efficiency and facilitate targeted processing , the effect of enhancing sensitivity

Active Publication Date: 2021-08-06
INST OF SOIL SCI CHINESE ACAD OF SCI
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Problems solved by technology

[0006] The mass spectrometry imaging effect of organic pollutant phthalates in plant tissues in the prior art is poor, and it is impossible to accurately describe its spatial distribution in plant tissues Problem, the present invention provides a kind of 2,5-dihydroxybenzoic acid as a mass spectrometry imaging target object and its mass spectrometry imaging method; by rationally setting the type of matrix and coating method, the organic pollutants in plant tissues can be effectively solved The imaging effect of phthalate mass spectrometry is poor, and it is impossible to accurately characterize its spatial distribution in plant tissues

Method used

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  • Mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as matrix and mass spectrum imaging method thereof
  • Mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as matrix and mass spectrum imaging method thereof
  • Mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as matrix and mass spectrum imaging method thereof

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Embodiment 1

[0046] This embodiment provides a target object for mass spectrometry imaging in which 2,5-dihydroxybenzoic acid is used as a matrix, and the target object includes phthalates in plant tissues.

[0047] This embodiment also provides a mass spectrometry imaging method in which 2,5-dihydroxybenzoic acid is used as a substrate, and the plant tissue adopts the tuber tissue cross section of carrot sample bis(2-ethylhexyl)phthalate (DEHP) space Distribution of mass spectrometry imaging, the experimental operation can refer to Figure 9 The operation process, the specific steps are as follows:

[0048] Step 1: Place the carrot tubers in liquid nitrogen for freezing for 1 minute; before slicing, place them in a -18°C environment for thawing for 5 minutes.

[0049] Step 2. Use a Leica CM1950 cryostat to slice the sample (Leica Microsystems, Wetzlar, Germany), set the room temperature of the slice to -15°C, and set the temperature of the knife head to -18°C to obtain carrot tissue slic...

Embodiment 2

[0056] This example provides a mass spectrometry imaging method in which 2,5-dihydroxybenzoic acid is used as a matrix. The operation method is basically the same as that of Example 1, the main difference is that in order to improve the chromatographic response of the target compound, reduce the impact of the matrix on the target compound. Interference, after the carrot tissue section is obtained in step 2 of Example 1, DEHP standard solution (10mg / mL, 10 μ L) is spot-coated on the surface of the carrot tissue section, and the carrot tissue section is adhered to the ITO-coated conductive glass slide and dried. Obtain dry and complete slices of carrot tissue.

[0057] Finally, the mass spectrometry data was processed by the data processing software to obtain the mass spectrometry imaging map of DEHP on the carrot tissue slice, and the center area of ​​DEHP was selected to be dotted, and the average ion intensity was calculated. The mass spectrogram was as follows Pic 4-1 shown...

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Abstract

The invention discloses a mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as a matrix and a mass spectrum imaging method thereof, which belong to the technical field of mass spectrum imaging. The method comprises the following steps: coating a matrix for assisting laser desorption ionization on a plant tissue containing the target, and putting the plant tissue into a mass spectrometer for ionization imaging. The target comprises phthalic acid ester in the plant tissue. According to the invention, the ionization efficiency of phthalic acid ester in the plant tissue can be effectively enhanced, and interference of the matrix on the target is effectively avoided, so that high-sensitivity mass spectrum imaging of in-situ distribution of phthalic acid ester in the plant tissue is realized, and identification and visual distribution analysis are carried out on the specific pollutant phthalic acid ester.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry imaging, and more specifically relates to a mass spectrometry imaging target object in which 2,5-dihydroxybenzoic acid is used as a substrate and a mass spectrometry imaging method thereof. Background technique [0002] Phthalic acid esters (PAEs) are used as plasticizers and modifying additives in the production process of plastic products, which can improve the plasticizers and modifying additives in the process of product processing and production, and can improve product strength and plasticity , is currently the world's largest output, the most widely used synthetic organic compounds. However, studies have shown that phthalates have environmental hormone effects, can interfere with the development of the reproductive system, and can induce teratogenic, carcinogenic, and mutagenic effects in the case of long-term high-dose pollution exposure. At present, 6 of the 14 phthalates used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/626G01N1/28
CPCG01N27/628G01N1/28G01N1/2813
Inventor 相雷雷王芳谷成刚卞永荣蒋新
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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