Mass spectrum imaging target taking 2, 5-dihydroxybenzoic acid as matrix and mass spectrum imaging method thereof
A technology of dihydroxybenzoic acid and mass spectrometry imaging, applied in the field of mass spectrometry imaging, can solve the problems of poor phthalate mass spectrometry imaging effect, inability to accurately describe the spatial distribution, etc., to enhance ionization efficiency and facilitate targeted processing , the effect of enhancing sensitivity
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Embodiment 1
[0046] This embodiment provides a target object for mass spectrometry imaging in which 2,5-dihydroxybenzoic acid is used as a matrix, and the target object includes phthalates in plant tissues.
[0047] This embodiment also provides a mass spectrometry imaging method in which 2,5-dihydroxybenzoic acid is used as a substrate, and the plant tissue adopts the tuber tissue cross section of carrot sample bis(2-ethylhexyl)phthalate (DEHP) space Distribution of mass spectrometry imaging, the experimental operation can refer to Figure 9 The operation process, the specific steps are as follows:
[0048] Step 1: Place the carrot tubers in liquid nitrogen for freezing for 1 minute; before slicing, place them in a -18°C environment for thawing for 5 minutes.
[0049] Step 2. Use a Leica CM1950 cryostat to slice the sample (Leica Microsystems, Wetzlar, Germany), set the room temperature of the slice to -15°C, and set the temperature of the knife head to -18°C to obtain carrot tissue slic...
Embodiment 2
[0056] This example provides a mass spectrometry imaging method in which 2,5-dihydroxybenzoic acid is used as a matrix. The operation method is basically the same as that of Example 1, the main difference is that in order to improve the chromatographic response of the target compound, reduce the impact of the matrix on the target compound. Interference, after the carrot tissue section is obtained in step 2 of Example 1, DEHP standard solution (10mg / mL, 10 μ L) is spot-coated on the surface of the carrot tissue section, and the carrot tissue section is adhered to the ITO-coated conductive glass slide and dried. Obtain dry and complete slices of carrot tissue.
[0057] Finally, the mass spectrometry data was processed by the data processing software to obtain the mass spectrometry imaging map of DEHP on the carrot tissue slice, and the center area of DEHP was selected to be dotted, and the average ion intensity was calculated. The mass spectrogram was as follows Pic 4-1 shown...
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