Product for noninvasively diagnosing male osteoporosis

Abstract

The invention discloses a product for noninvasively diagnosing male osteoporosis. The product can detect the expression level of UTY in the blood of a subject, so that whether the subject is an osteoporosis patient can be judged according to the expression level of the UTY. The method of the product for diagnosing male osteoporosis is simple, the diagnosing can be accomplished by drawing a small amount of blood of the subject, so that the pain of the subject can be reduced; and the product is accurate and reliable in detection result.

Description

technical field

[0001] The invention belongs to the field of biomedicine and relates to a product for non-invasive diagnosis of male osteoporosis. Background technique

[0002] Osteoporosis is a common disease among middle-aged and elderly people, and the incidence of osteoporosis in men is on the rise. Compared with women, osteoporosis occurs earlier and faster in men. Alcoholism, hypogonadism, and smoking are major risk factors for osteoporosis in men.

[0003] Clinical symptoms caused by osteoporosis include trunk shortening, hunchback, and low back pain; limb bones are prone to fractures, and femoral neck fractures are the most common. These early symptoms are not very obvious, if left unchecked, the adverse consequences will be very serious, 50% of people will be disabled for life.

[0004] Pain is the most common symptom of primary osteoporosis. Low back pain is more common. However, many middle-aged men often mistake it for fatigue when they feel discomfort in their...

Examples

Embodiment 1

[0032] Example 1 QPCR detection of differentially expressed genes

[0033] 1. Sample collection

[0034] A total of 30 male patients with osteoporosis were selected, and the inclusion criteria were: T<-2.5 of bone mineral density measured by dual-energy X-ray; T<-2.5 with previous history of fragility fracture; normal liver and kidney function. Exclusion criteria: ① Those who used anti-osteoporosis drugs in the past; ② Diabetes, thyroid or parathyroid disease, endocrine diseases such as adrenal gland and gonad; ③ Secondary osteoporosis. The average age is 78 years old. The peripheral blood of the above-mentioned patients was extracted as the experimental sample, and the peripheral blood of 30 normal persons was collected as the control sample.

[0035] 2. Validation at the mRNA level

[0036] 1. Total RNA extraction

[0037] Use the RNAprep Pure High-efficiency Blood Total RNA Extraction Kit (Cat. No.: DP443) from Tiangen Company to extract blood total RNA, and follow the ...

Embodiment 2

[0102] Preparation of Example 2 Male Osteoporosis Diagnostic Kit

[0103] According to the correlation between UTY gene and male osteoporosis, male osteoporosis can be diagnosed by detecting the expression of UTY gene. Accordingly, the present invention provides a method for diagnosing male osteoporosis based on detecting UTY gene expression. A kit, the components in the diagnostic kit are as follows: a SYBR Green polymerase chain reaction system; a pair of primers for amplifying UTY gene and GAPDH gene. The forward primer sequence for amplifying UTY gene is 5'-AACCTCAAGATGCCATTA-3', the reverse primer sequence is 5'-CCACCATTCCAGTTATCA-3'; the forward primer sequence for amplifying GAPDH is 5'-GTGGACCTGACCTGCCGTCT-3', reverse The primer sequence was 5'-GGAGGAGTGGGTGTCGCTGT-3'. The SYBR Green polymerase chain reaction system includes PCR buffer, dNTPs, and SYBR Green fluorescent dye. PCR buffer composition: 25mM KCL, 2.5mM MgCL 2 , 200mM (NH 4 ) 2 SO 4 .

technical field

[0001] The invention belongs to the field of biomedicine and relates to a product for non-invasive diagnosis of male osteoporosis. Background technique

[0002] Osteoporosis is a common disease among middle-aged and elderly people, and the incidence of osteoporosis in men is on the rise. Compared with women, osteoporosis occurs earlier and faster in men. Alcoholism, hypogonadism, and smoking are major risk factors for osteoporosis in men.

[0003] Clinical symptoms caused by osteoporosis include trunk shortening, hunchback, and low back pain; limb bones are prone to fractures, and femoral neck fractures are the most common. These early symptoms are not very obvious, if left unchecked, the adverse consequences will be very serious, 50% of people will be disabled for life.

[0004] Pain is the most common symptom of primary osteoporosis. Low back pain is more common. However, many middle-aged men often mistake it for fatigue when they feel discomfort in their...

Examples

Embodiment 1

[0032] Example 1 QPCR detection of differentially expressed genes

[0033] 1. Sample collection

[0034] A total of 30 male patients with osteoporosis were selected, and the inclusion criteria were: T<-2.5 of bone mineral density measured by dual-energy X-ray; T<-2.5 with previous history of fragility fracture; normal liver and kidney function. Exclusion criteria: ① Those who used anti-osteoporosis drugs in the past; ② Diabetes, thyroid or parathyroid disease, endocrine diseases such as adrenal gland and gonad; ③ Secondary osteoporosis. The average age is 78 years old. The peripheral blood of the above-mentioned patients was extracted as the experimental sample, and the peripheral blood of 30 normal persons was collected as the control sample.

[0035] 2. Validation at the mRNA level

[0036] 1. Total RNA extraction

[0037] Use the RNAprep Pure High-efficiency Blood Total RNA Extraction Kit (Cat. No.: DP443) from Tiangen Company to extract blood total RNA, and follow the ...

Embodiment 2

[0102] Preparation of Example 2 Male Osteoporosis Diagnostic Kit

[0103] According to the correlation between UTY gene and male osteoporosis, male osteoporosis can be diagnosed by detecting the expression of UTY gene. Accordingly, the present invention provides a method for diagnosing male osteoporosis based on detecting UTY gene expression. A kit, the components in the diagnostic kit are as follows: a SYBR Green polymerase chain reaction system; a pair of primers for amplifying UTY gene and GAPDH gene. The forward primer sequence for amplifying UTY gene is 5'-AACCTCAAGATGCCATTA-3', the reverse primer sequence is 5'-CCACCATTCCAGTTATCA-3'; the forward primer sequence for amplifying GAPDH is 5'-GTGGACCTGACCTGCCGTCT-3', reverse The primer sequence was 5'-GGAGGAGTGGGTGTCGCTGT-3'. The SYBR Green polymerase chain reaction system includes PCR buffer, dNTPs, and SYBR Green fluorescent dye. PCR buffer composition: 25mM KCL, 2.5mM MgCL 2 , 200mM (NH 4 ) 2 SO 4 .

Images