A strain of Pseudomonas putida degrading coumarin and its application

A technology of Pseudomonas putida and coumarin, which is applied in the field of bioengineering, can solve the problems of carcinogenicity and limit development and use, and achieve the effect of eliminating carcinogenic factors

Active Publication Date: 2021-02-19
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the main component of tonka bean tincture, coumarin has a strong carcinogenic effect on the human body, which greatly limits its development and use in the fields of food, cosmetics and tobacco

Method used

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  • A strain of Pseudomonas putida degrading coumarin and its application
  • A strain of Pseudomonas putida degrading coumarin and its application
  • A strain of Pseudomonas putida degrading coumarin and its application

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Embodiment Construction

[0019] In order to make the objects and advantages of the present invention clearer, the present invention will be further described below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] The experimental materials of this application are as follows:

[0021] Recipe for Coumarin Medium:

[0022] K2HPO4 1 g / L, MgSO4 7H2O 0.5 g / L, FeSO4 7H2O 0.005 g / L, NaCl 0.5 g / L, KH2PO4 0.65 g / L, MnSO4 0.001 g / L, (NH4)2 SO4 0.5 g / L , CaCl2•2H2O 0.1g / L, Na2MoO4•2H2O 0.005 g / L, add 2 g / L coumarin on the basis of this medium.

[0023] Published formula of bacterial LB medium:

[0024] 10g tryptone, 5g yeast extract, 10g NaCl, 1L distilled water, pH to 7.0.

[0025] 1. Screening of coumarin-degrading bacteria

[0026] Select 1-3 pieces of soil under representative walnut, persimmon, and corn plants in Shuiniuzhang Village, High-tech Zone, Zhengzho...

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Abstract

The invention relates to the field of coumarin-degrading bacteria, in particular to a coumarin-degrading bacteria and its application. The classification of described coumarin-degrading bacteria is called Pseudomonas putida ( Pseudomonas putida HS‑C2), which was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on May 13, 2019, with the preservation number CGMCC No.17758. Pseudomonas putida can degrade coumarin and generate dihydrocoumarin, and the degradation rate of coumarin is 99.94%.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a strain of Pseudomonas putida for degrading coumarin and its application, in particular to a strain of Pseudomonas putida and its culture medium, as well as a strain of Pseudomonas putida for degrading coumarin and generating dihydrocoumarin application. Background technique [0002] Coumarin was discovered in 1820 and widely exists in higher plants, especially plants such as Fabaceae, Rutaceae, Orchidaceae, Compositae, Solanaceae, Umbelliferae, and Saxifragaceae, and even neutralizes microorganisms in animals Some coumarins were also found in metabolites. Coumarin can be regarded as a lactone of o-hydroxycinnamic acid, which has an aromatic smell. Coumarin is often divided into simple coumarin, pyranocoumarin, and furanocoumarin according to the different substituents on the ring and their positions. and other coumarins, etc. [0003] Coumarin, as a spice and fixative, can be add...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C12P17/06A23L29/00A23L5/20C12R1/40
CPCA23L5/28A23L29/065C12N1/20C12P17/06C12N1/205C12R2001/40
Inventor黄申毛多斌杨鹏飞韩丽杨靖杨峰马宁周利峰魏涛闫美玲卢慧
OwnerZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY