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Method for detecting whether African swine fever virus carried in soil of pig farm or not

A technology for African swine fever virus and pig farms, which is applied in the direction of microbial-based methods, biochemical equipment and methods, and microbial measurement/inspection. Infection loss and other problems, to achieve the effect of delaying the resumption of pig farm production, saving operating costs, and simple detection methods

Active Publication Date: 2019-09-20
湖南省动物疫病预防控制中心 +1
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the stable characteristics of African swine fever virus, it brings serious challenges to the resumption of production in pig farms. Once the disinfection is not complete, there will be surviving viruses in the pig farm environment or soil, and the probability of reinfection after resumption of production is 100%.
[0005] Usually, the treatment of the epidemic area will adopt repeated cleaning, cleaning, disinfection, and vacancy. This series of measures can effectively kill the African swine fever virus on the surface of the breeding place, but the virus in the soil cannot be effectively treated. It has been shown that, African swine fever virus can penetrate into the soil with water flow, urine, feces, etc., causing the epidemic to spread
Therefore, once there is unkilled African swine fever virus in the soil before the pig farm resumes production, it will pose a serious threat to the pig farm after the resumption of production, and even cause losses due to re-infection
[0006] Therefore, how to accurately determine whether there is African swine fever virus in the breeding environment, when to lift the blockade of the epidemic area, and reasonably grasp the time of resumption of production is of great significance to the pig breeding industry. However, there is currently no good method to directly detect the environment of pig farms, especially African swine fever virus in the soil, and there is no accurate method to reasonably control the resumption time of pig farms after the onset of the disease. The resumption of production of pig farms in affected areas depends entirely on experience and luck

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  • Method for detecting whether African swine fever virus carried in soil of pig farm or not
  • Method for detecting whether African swine fever virus carried in soil of pig farm or not
  • Method for detecting whether African swine fever virus carried in soil of pig farm or not

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Embodiment 1

[0038] A method for detecting whether African swine fever virus is carried in the soil of a pig farm, including putting earthworms in the surrounding area of ​​pig houses or in the polluted production area or the place where sick and dead pigs are treated or in the soil at the entrance and exit of pig farms for cultivation, and then detecting whether the earthworms are inside or outside the pig farm. Steps to carry African swine fever virus.

[0039] In this method, earthworms are first put into the soil around the pig house or around the production pig house or in the polluted production area or the place where sick and dead pigs are treated, or at the entrance and exit of the pig farm. The method is simple and easy, has low detection cost and high efficiency, and can effectively prevent farms from being infected with African swine fever virus.

Embodiment 2

[0041] A method for detecting whether African swine fever virus is carried in pig farm soil, specifically comprising the following steps:

[0042] (1) Selection and treatment of earthworm release sites: Select the entrances and exits of live pig farms around the pig house or production polluted area or where sick and dead pigs are treated as the site to be tested, and pour thoroughly with sterilized purified water;

[0043] (2) Earthworm release: put earthworms into the place to be tested, and then irradiate the ground with a low-power ultraviolet lamp;

[0044](3) Earthworm recovery: Cultivate for 3-5 days after putting in, dig out or lure out earthworms according to the location of earthworm feces, place earthworms separately in different areas, and mark them well, the number of earthworms recovered is greater than 70% of the total number of put in;

[0045] (4) Detection: After crushing the recovered earthworms, use the fluorescent PCR method to detect whether they carry Af...

Embodiment 3

[0047] A method for detecting whether African swine fever virus is carried in pig farm soil, specifically comprising the following steps:

[0048] (1) Selection and treatment of earthworm release sites: Select the surrounding areas of a diseased pig house, production polluted area, and the location of sick and dead pigs announced by the Ministry of Agriculture and Rural Affairs as the site to be tested, and use sterilized purified water to soak the soil surface; test time One week after the outbreak of African swine fever in the farm, the daily average temperature was determined to be 15-20°C, which is suitable for the growth of earthworms; 2 Mark the size of the test area, and mark a total of 10 detection areas, numbered sequentially from 1-10. The detection area, detection position, and input amount of earthworms are shown in Table 1 below;

[0049] (2) Earthworm feeding: according to the number of 10-15 earthworms per square meter, a total of 560 earthworms were put in. Bef...

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Abstract

The invention belongs to the technical field of livestock disease detection and particularly relates to a method for detecting whether African swine fever virus carried in soil of a pig farm or not. A detection process of the method includes steps: culturing earthworms in soil around a pig house, or a production sewage area, or a position for treatment of pigs dying from diseases or an access of the pig farm, and then detecting whether the African swine fever virus is carried inside and outside earthworm bodies or not. The method is high in detection efficiency and accuracy, and risks of the farm can be anticipated, so that loss reduction is realized; aiming at the pig farm in an area with African swine fever virus infection, an important basis can be provided for production recovery in the pig farm to reduce the risks of re-infection after production recovery; in addition, a scientific basis is provided for daily disinfection management and the like in the pig farm.

Description

technical field [0001] The invention belongs to the technical field of livestock disease detection, and in particular relates to a method for detecting whether African swine fever virus is carried in pig farm soil. Background technique [0002] African swine fever (African swine fever virus, ASFV) is an acute, fever-infectious, highly contagious virus-induced swine disease. It is characterized by a short course of disease, but the mortality rate is as high as 100%. Fever, cyanosis of the skin, obvious bleeding of lymph nodes, kidneys, and gastrointestinal mucosa. [0003] From August 3rd to 15th, 2018, three African swine fever outbreaks were discovered one after another in China in Shenyang, Liaoning, Zhengzhou, Henan, and Lianyungang, Jiangsu, which were far apart. On August 19, an African swine fever epidemic was confirmed in a farm in Haizhou District, Lianyungang City, Jiangsu Province, with 615 cases and 88 deaths. On August 24, the Animal Husbandry and Veterinary Bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686A01K67/033C12R1/93
CPCA01K67/0332C12Q1/686C12Q1/701C12Q2563/107
Inventor 何世成王昌建刘道新唐小明喻正军胡巧云周小双
Owner 湖南省动物疫病预防控制中心
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