Method for detecting whether African swine fever virus carried in soil of pig farm or not

Abstract

The invention belongs to the technical field of livestock disease detection and particularly relates to a method for detecting whether African swine fever virus carried in soil of a pig farm or not. A detection process of the method includes steps: culturing earthworms in soil around a pig house, or a production sewage area, or a position for treatment of pigs dying from diseases or an access of the pig farm, and then detecting whether the African swine fever virus is carried inside and outside earthworm bodies or not. The method is high in detection efficiency and accuracy, and risks of the farm can be anticipated, so that loss reduction is realized; aiming at the pig farm in an area with African swine fever virus infection, an important basis can be provided for production recovery in the pig farm to reduce the risks of re-infection after production recovery; in addition, a scientific basis is provided for daily disinfection management and the like in the pig farm.

Description

technical field [0001] The invention belongs to the technical field of livestock disease detection, and in particular relates to a method for detecting whether African swine fever virus is carried in pig farm soil. Background technique [0002] African swine fever (African swine fever virus, ASFV) is an acute, fever-infectious, highly contagious virus-induced swine disease. It is characterized by a short course of disease, but the mortality rate is as high as 100%. Fever, cyanosis of the skin, obvious bleeding of lymph nodes, kidneys, and gastrointestinal mucosa. [0003] From August 3rd to 15th, 2018, three African swine fever outbreaks were discovered one after another in China in Shenyang, Liaoning, Zhengzhou, Henan, and Lianyungang, Jiangsu, which were far apart. On August 19, an African swine fever epidemic was confirmed in a farm in Haizhou District, Lianyungang City, Jiangsu Province, with 615 cases and 88 deaths. On August 24, the Animal Husbandry and Veterinary Bu...

AI Technical Summary

Problems solved by technology

Due to the stable characteristics of African swine fever virus, it brings serious challenges to the resumption of production in pig farms. Once the disinfection is not complete, there will be surviving viruses in the pig farm environment or soil, and the probability of reinfection after resumption of production is 100%.

[0005] Usually, the treatment of the epidemic area will adopt repeated cleaning, cleaning, disinfection, and vacancy. This series of measures can effectively kill the African swine fever virus on the surface of the breeding place, but the virus in the soil cannot be effectively treated. It has been shown that, African swine fever virus can penetrate into the soil with water flow, urine, feces, etc., causing the epidemic to spread

Therefore, once there is unkilled African swine fever virus in the soil before the pig farm resumes production, it will pose a serious threat to the pig farm after the resumption of production, and even cause losses due to re-infection

[0006] Therefore, how to accurately determine whether there is African swine fever virus in the breeding environment, when to lift the blockade of the epidemic area, and reasonably grasp the time of resumption of production is of great significance to the pig breeding industry. However, there is currently no good method to directly detect the environment of pig farms, especially African swine fever virus in the soil, and there is no accurate method to reasonably control the resumption time of pig farms after the onset of the disease. The resumption of production of pig farms in affected areas depends entirely on experience and luck

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