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Strain of streptomycete CCPM7649 with strong anticancer activity and application thereof
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A technology of CCPM7649, anti-cancer activity, applied in the field of biopharmaceuticals, can solve the problem of large side effects of anti-cancer drugs
Active Publication Date: 2019-09-27
深圳市人田谷生物医药科技有限公司
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[0003] In order to solve the problem of large side effects of existing anticancer drugs, the present invention provides a strain of Streptomyces CCPM7649 with strong anticancer activity and its application
Method used
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Embodiment 1
[0029] A strain of Streptomyces CCPM7649 with strong anticancer activity, belonging to the genus Streptomyces (Streptomyces), representing a previously unknown bacterial species (species), has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No. 17112, the preservation date is January 4, 2019, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.
Embodiment 2
[0031] This example provides a screening method for Streptomyces CCPM7649.
[0032] 1. Preparation method of screening medium and related solutions:
[0034] Potato flour or other starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, remember to shake well after high pressure.
[0035] (2) Potassium dichromate: The final concentration of the solution is 50mg / L. The concentration of the storage solution is: 5g / L, the solvent is water, and it is prepared immediately after use, protected from light, and sterilized by filtration. Toxic, do not touch the skin or inhale; when preparing 100mL GRC001 solid medium, add 1mL potassium dichromate stock solution, add it when the medium is not too hot, and mix well.
[0036] (3) FeSO 4 ·7H 2 O mother liquor: 0.01 g / mL. 1g FeSO 4 ·7H 2 O dissolved in 100mLH 2 In O, add 1 ...
Embodiment 3
[0045] The present embodiment provides a kind of amplification method of Streptomyces CCPM7649:
[0046] Take a single colony of Streptomyces CCPM7649 from the plate and put it into 5mL GRC001 liquid medium, 160 rpm, 14-42°C, after amplification for 3-18 days, transfer 5mL bacterial liquid to 100mL GRC001 liquid medium, 160 Rotation per minute, 14-42°C, large-scale amplification for 3-18 days, and finally the bacterial liquid was centrifuged at 8000rpm / min for 10min, and the supernatant was taken for subsequent anti-cancer experiments.
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Abstract
The invention relates to a strain of streptomycete CCPM7649 with strong anticancer activity and application thereof, and belongs to the technical field of biopharmaceuticals. In order to solve the problem of large side effects of the conventional anticancer drugs, the invention provides the strain of streptomycete CCPM7649 with strong anticancer activity, which belongs to the streptomyces, and has been preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 17112. The strain is derived from a human intestinal tract, and is a newly discovered actinomycete that generates metabolites with anticancer activity, and the metabolites can be used for treating cancer. Being found in the human intestine tract, the strain is low in or is free of side effects on the human body, thereby solving the problems of large side effects of the current anticancer drugs and poor patient compliance, and the purpose of preventing cancer by regulating the balance of intestinal flora is expected to achieve.
Description
technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a strain of Streptomyces CCPM7649 with strong anticancer activity and its application. Background technique [0002] Cancer is a disease caused by the abnormality of the mechanism of controlling cell growth and proliferation caused by the combination of genetic and environmental factors. The morbidity and mortality are increasing year by year, which seriously threatens human health and consumes social resources. At present, the main treatment methods are surgery, chemotherapy, radiotherapy and immunotherapy. Although surgical treatment can remove tumors, it is ineffective for certain types of cancer, such as leukemia and cancers that have metastasized throughout the body, and any type of cancer cannot be treated surgically when it reaches an advanced stage. The current chemotherapy and radiotherapy methods will cause serious harm to the human body, and are...
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