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A Streptomyces ccpm7649 with potent anticancer activity and its application
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A CCPM7649, anti-cancer activity technology, applied in the field of biopharmaceuticals, can solve the problem of large side effects of anti-cancer drugs
Active Publication Date: 2020-09-22
深圳市人田谷生物医药科技有限公司
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[0003] In order to solve the problem of large side effects of existing anticancer drugs, the present invention provides a strain of Streptomyces CCPM7649 with strong anticancer activity and its application
Method used
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Embodiment 1
[0029] A strain of Streptomyces CCPM7649 with strong anticancer activity, belonging to the genus Streptomyces (Streptomyces), representing a previously unknown bacterial species (species), has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No. 17112, the preservation date is January 4, 2019, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.
Embodiment 2
[0031] This example provides a screening method for Streptomyces CCPM7649.
[0032] 1. Preparation method of screening medium and related solutions:
[0034] Potato flour or other starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, remember to shake well after high pressure.
[0035] (2) Potassium dichromate: The final concentration of the solution is 50mg / L. The concentration of the storage solution is: 5g / L, the solvent is water, and it is prepared immediately after use, protected from light, and sterilized by filtration. Toxic, do not touch the skin or inhale; when preparing 100mL GRC001 solid medium, add 1mL potassium dichromate stock solution, add it when the medium is not too hot, and mix well.
[0036] (3) FeSO 4 ·7H 2 O mother liquor: 0.01 g / mL. 1g FeSO 4 ·7H 2 O dissolved in 100mLH 2 In O, add 1 ...
Embodiment 3
[0045] The present embodiment provides a kind of amplification method of Streptomyces CCPM7649:
[0046] Take a single colony of Streptomyces CCPM7649 from the plate and put it into 5mL GRC001 liquid medium, 160 rpm, 14-42°C, after amplification for 3-18 days, transfer 5mL bacterial liquid to 100mL GRC001 liquid medium, 160 Rotation per minute, 14-42°C, large-scale amplification for 3-18 days, and finally the bacterial liquid was centrifuged at 8000rpm / min for 10min, and the supernatant was taken for subsequent anti-cancer experiments.
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Abstract
The invention relates to a strain of streptomyces CCPM7649 with strong anticancer activity and its application, belonging to the technical field of biopharmaceuticals. In order to solve the problem of large side effects of existing anticancer drugs, the present invention provides a strain of Streptomyces CCPM7649 with strong anticancer activity, belonging to the genusStreptomyces, which has been preserved in the General Microorganism Center of China Microbiological CultureCollection Management Committee. The number is CGMCC No.17112. This bacterium is derived from the human intestinal tract. It is a newly discovered actinomycete. It can produce metabolites with anti-cancer activity, and its metabolites can be used to treat cancer. Since the bacterium is found in the human intestinal tract, it has very low or no side effects on the human body, which solves the problems of large side effects of current anticancer drugs and poor patient compliance, and is expected to prevent cancer by regulating the balance of intestinal flora Purpose.
Description
technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a strain of Streptomyces CCPM7649 with strong anticancer activity and its application. Background technique [0002] Cancer is a disease caused by the abnormality of the mechanism of controlling cell growth and proliferation caused by the combination of genetic and environmental factors. The morbidity and mortality are increasing year by year, which seriously threatens human health and consumes social resources. At present, the main treatment methods are surgery, chemotherapy, radiotherapy and immunotherapy. Although surgical treatment can remove tumors, it is ineffective for certain types of cancer, such as leukemia and cancers that have metastasized throughout the body, and any type of cancer cannot be treated surgically when it reaches an advanced stage. The current chemotherapy and radiotherapy methods will cause serious harm to the human body, and are...
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