Composite amplification kit for simultaneously detecting autosomal and Y chromosome STR loci

Abstract

The invention discloses a composite amplification kit for simultaneously detecting autosomal and Y chromosome STR loci. The kit at least comprises specific amplification primer pairs of 40 loci, and the sequences of the primer pairs include SEQ ID NO.1 to SEQ ID NO.78. The kit can simultaneously detect 20 Y chromosome STR loci, 19 autosomal STR loci and Amelogenin locus, not only is accurate in typing, but also shortens the detection time, and improves the efficiency of removing and determining criminal suspects.

Description

technical field [0001] The invention belongs to the field of analytical genetics, relates to a multiple amplification test system for human chromosome STR loci, in particular to a multiple amplification kit for simultaneously detecting autosome and Y chromosome STR loci. Background technique [0002] Since the 1990s, DNA-STR typing technology has become one of the main means of forensic evidence testing due to its advantages of good specificity, high sensitivity, simple and fast typing, and easy standardization. Currently commonly used STR detection kits include autosomal STR detection kits and Y chromosome STR detection kits, which can be used for individual identification detection and paternity detection, respectively. When a major criminal case occurs, the autosomal STR test is often performed on the physical evidence extracted at the scene first, and when it is confirmed that it is the physical evidence left by a male criminal suspect, the Y chromosome STR test is perfo...

AI Technical Summary

Problems solved by technology

[0003] At present, the Y chromosome STR kit (Yfiler Plus) and the autosomal STR kit (Identifiler) produced by ABI Company, which are most used in China, are affected by factors such as the status of the sample, the number of amplification sites, and the amplification system. The two PCR amplification processes take 5-6 hours, so the two inspection procedures often delay the best chance of catching the suspect because of the increased detection time, and also because of the physical evidence left by the suspect at the scene. The small amount leads to detection failure, and eventually leads to the loss of investigation clues

[0004] All in all, with the increase of criminal cases, the existing detection kits can no longer meet the requirements of public security for the rapid detection and on-site detection of forensic DNA in actual combat. How to realize the detection of autosomal STR loci and Y chromosome STR loci together , to shorten the detection time while improving the efficiency of excluding and identifying criminal suspects has become a problem to be solved

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