Kit for detecting trace drugs in sewage by using time-resolved fluorescence immunochromatography technology, and application of kit

Abstract

The invention discloses a kit for detecting trace drugs in sewage by using a time-resolved fluorescence immunochromatography technology, and an application of the kit. The kit comprises a kit body; and an aptamer affinity column, a time-resolved fluorescent microsphere freeze-drying tube and an immunochromatographic test strip are arranged in the kit body. According to the kit, a time-resolved fluorescent microsphere is used for marking an antibody, the half-life period of fluorescence is long, the product stability is relatively high, and the normal-temperature storage is facilitated; the detection limit reaches 50ng / L, so that accurate detection of the drugs is realized; small molecules such as the to-be-detected drugs, namely, methylamphetamine and the like in the sewage are enriched and concentrated through the aptamer affinity column, so that the sewage detection sensitivity of the test strip is improved, and the practical sewage detection sensitivity can reach 5ng / L according tothe effect of concentration by 10 times; the kit has obvious advantages in the aspects of inter-assay variations and the precision of the test strip, and is sufficient in reaction and higher in sensitivity; the operation is simple and convenient; and the test strip is simple and convenient to use and operate, and does not need to be operated by professionals.

Description

technical field [0001] The invention relates to a kit for detecting trace narcotics in sewage by time-resolved fluorescent immunochromatography technology and an application thereof, belonging to the field of detection of trace narcotics in sewage. Background technique [0002] Drugs seriously endanger human health, affect family harmony, threaten social security, and damage national economy, which has become a common problem that the international community needs to face today. In order to fight drugs, governments of various countries have invested a lot of money. my country has also invested a lot of manpower, material and financial resources in rescuing and treating drug addicts, carrying out anti-drug education and scientific research, and increasing anti-drug efforts. Accurately grasping drug abuse information plays an important role in combating drug crimes, guiding the development of drug prevention, comprehensively carrying out various anti-drug work, and reducing t...

AI Technical Summary

Problems solved by technology

Currently, the immunochromatography technology provided by the market for rapid detection of products has low sensitivity, large batch-to-batch differences, poor stability, and short product shelf life.

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