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Method for quantifying stevioside by potassium ferricyanide-luminol chemiluminescence system

A steviol glycoside and chemiluminescence technology, which is applied in the detection field, can solve problems such as the unseen chemiluminescence determination of steviol glycosides, and achieve the effects of novel design, remarkable innovation and simple operation.

Inactive Publication Date: 2009-04-08
济宁奥星甜菊制品有限公司
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Problems solved by technology

So far (April 28, 2006, information center of Heilongjiang Science and Technology Information Bureau checked for novelty), there is no report on the determination of steviol glycosides by chemiluminescence

Method used

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  • Method for quantifying stevioside by potassium ferricyanide-luminol chemiluminescence system

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Embodiment Construction

[0027] 1 Instrument and equipment

[0028] 1.1 MPI-B multi-parameter chemiluminescence analysis and testing system (Xi'an Ruimai Electronics Co., Ltd.). Sampling, sample injection, experimental data collection and processing in the whole analysis process are all completed by MPI-B multi-parameter chemiluminescence analysis and test system software under Windows XP system.

[0029] 1.2UV-1600 ultraviolet-visible spectrophotometer: Beijing Rayleigh Analytical Instrument Company.

[0030] 2 Materials and reagents

[0031] 2.1 Materials: fresh stevia leaves, cultivated in this laboratory; steviol glycosides, Harbin Luye Technology Co., Ltd.

[0032] 2.2 Reagents: (1) Use 0.1mol / L NaOH as solvent to prepare different concentrations of Fe(CN) 3- 6 . 3-aminophthaloyl cyclic hydrazine (Luminol) is the product of Sigma Company (Na with pH value of 9.2 2 CO 3 -NaHCO 3 buffer solution preparation). (2) Standard solution of steviol glycosides (10mg / mL): weigh steviol glycosides a...

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Abstract

The invention provides a method for quantitating stevioside in potassium ferricyanide-luminol chemoluminescence system. The method comprises making Fe(CN)3-6 solution, Luminol solution and stevioside standard solution with different concentration; employing spectrophotometer to perform full wavelength scanning on the stevioside standard solution between 190-1100nm; in the active pump, three-way mixing the carrier Luminol and stevioside standard solution at 45r / min flow speed via corresponding pipes and inputting to an analysis system; the slave pump injecting the Fe(CN)3-6 solution at 35r / min flow speed into the carrier flow via sixteen-way injection valve to mix with the carrier flow; the K3Fe(CN)6-Luminol is a margin luminary system (l0), sample enhancing signal (IS), getting peak value ( deltaI=IS-I0) to quantitate and the luminous intensity is displayed with number.

Description

(1) Technical field [0001] The invention designs a detection method, specifically a detection method of steviol glycosides. (2) Background technology [0002] At present, the number of diabetic patients is gradually increasing. In addition, excessive intake of absorbable sugar by normal people is not conducive to health. Therefore, the development and utilization of sweeteners that have a taste similar to sucrose and are non-absorbable sugars are the future development direction. [0003] Among the major categories of sweeteners allowed to be added in the world, half come from chemical synthesis, and the other half come from natural plants. With the continuous improvement of people's living standards, more and more people tend to use natural plant energy-free sweeteners. [0004] Among the natural plant non-energy sweeteners, the stevia plant has a relatively high sweetness, and its sugar has been used as a sweetener in the past ten years. There are 7 sweet components in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 郝再彬杨丹苍晶王贵民一井真比古
Owner 济宁奥星甜菊制品有限公司
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