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A gene zmcaspl2b2 regulating the wax structure of corn leaf epidermis and its encoded protein and application

A technology of epidermal wax and corn leaves, applied in the field of plant genetic engineering, can solve the problems of few reports on the function research of CASPLs gene family

Active Publication Date: 2021-04-27
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, so far, there are few reports on the function of CASPLs gene family

Method used

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  • A gene zmcaspl2b2 regulating the wax structure of corn leaf epidermis and its encoded protein and application
  • A gene zmcaspl2b2 regulating the wax structure of corn leaf epidermis and its encoded protein and application
  • A gene zmcaspl2b2 regulating the wax structure of corn leaf epidermis and its encoded protein and application

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Experimental program
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Effect test

Embodiment 1

[0040] 1) Observation of mutant phenotypes

[0041] The zmcaspl2b2 mutant comes from a natural mutation in the field, and its background material is the maize inbred line LY8405. At the seedling stage, the shape of the zmcaspl2b2 mutant and LY8405 was similar. However, with the growth and development of the struts, the zmcaspl2b2 mutant showed a curled and erect phenotype of leaves, and this phenotype was most obvious at the adult plant stage. figure 1 (a)- figure 1 (c). In order to describe the rolling leaf phenotype, the natural leaf width (NLW) and maximum leaf width (MLW) at panicle position of LY8405 and zmcaspl2b2 were measured, and the leaf rolling index (LRI, NLW / MLW) was obtained, and the statistical results were shown in the zmcaspl2b2 mutant , the above three traits were significantly decreased figure 1 (d)- figure 1 (f), showing that the zmcaspl2b2 mutant exhibits narrowed and curled leaf phenotypes. In addition to the structure of leaves, the growth and devel...

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Abstract

The invention provides a gene ZmCASPL2B2 for regulating the wax structure of corn leaf epidermis and its encoded protein and its application, belonging to the technical field of plant genetic engineering. The nucleotide sequence of the gene ZmCASPL2B2 is shown in SEQ ID No.1. The amino acid sequence of the protein is shown in SEQ ID No.2; the ZmCASPL2B2 gene has the function of regulating the waxy structure of the corn leaf epidermis. By regulating the waxy structure of the leaf epidermis, it affects the loss of water on the leaf surface and the resistance to abiotic stress ; The present invention firstly determines the function of the ZmCASPL2B2 gene by means of genetic engineering combined with physiological and biochemical tests, and provides valuable genetic resources for maize breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a gene ZmCASPL2B2 for regulating the waxy structure of corn leaf epidermis and its encoded protein and application. Background technique [0002] Drought and salt stress are two major abiotic stresses affecting plant production. Plants mainly respond to these stresses by changing metabolism, physiology, and growth and development to minimize the impact of these stresses on their own growth and development (Qin, T. and Zhao, H. (2017) A Nucleus-Localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance. 175, 1321-1336). Since both drought stress and salt stress can cause cell dehydration and osmotic stress, the mechanisms by which plants respond to these two stresses are quite similar, mainly including; Moisture (Xiong, L., Wang, R.G., Mao, G. and Koczan, J.M. (2006) Identification of drought tolerance determinants by genetic analysis ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 邱法展潘振远刘敏谭增栋肖姿仪
Owner HUAZHONG AGRI UNIV
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