Method for extracting DNA of schistosome from feces and rapid identification LF-RPA method of method

Abstract

The invention discloses a method for extracting DNA of schistosome from feces and a rapid identification LF-RPA method of the method. According to the method for extracting the DNA of the schistosomefrom the feces, diagnosing is carried out by extracting parasite egg DNA, and the detection result can be recognized by naked eye of an unprofessional person without any training, the method can be popularized and used in grassroots units or backward areas in remote areas, and an established diagnosing method has good sensitivity, specificity and ability to distinguish schistosoma japonicum, schistosoma mansoni and orientobilharzia, has advantages in the diagnosis of low-intensity infections, and has great significance for the prevention and treatment of infection of schistosome and other flukes.

Description

technical field [0001] The invention relates to the technical field of parasite detection, in particular to a method for extracting schistosome DNA from feces, and an LF-RPA method for rapid identification of the obtained DNA and its application. Background technique [0002] Schistosomiasis is one of the oldest parasitic diseases in humans, and the progress in controlling schistosomiasis is still slow in recent years. The traditional diagnostic methods of schistosomiasis include etiological diagnosis and immunological diagnosis. Etiological diagnosis methods, such as feces detection, miracidium hatching, etc., have a low detection rate of pathogen detection, and infection often needs to reach a certain level before miracidia can be hatched from feces or eggs can be collected. It is difficult to detect and has a high rate of missed detection. A detection takes 4-5 hours, which takes a long time and is relatively insensitive. It cannot meet the requirements of low-endemic are...

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Problems solved by technology

The sensitivity is higher than that of etiological diagnosis, which reduces the missed detection rate to a certain extent. Compared with the etiological method, it is simple and fast, and has the advantages of high specificity, strong sensitivity, rapid response, and simple operation. However, it is generally difficult to distinguish before and after treatment. After treatment, the antibody level in the animal body remains high. With IHA and ELISA detection, it is easy to repeatedly diagnose positive results and lead to repeated treatment, and it has high cross-reactivity with other parasite diagnoses.

[0003] With the rapid development of molecular biology, more and more molecular biology detection techniques have been developed and applied by scientific researchers. Molecular biology detection techniques are based on the amplification of specific biological macromolecules (nucleic acid, protein). The molecular biology diagnosis of the disease has been relatively thorough, and the DNA of schistosomiasis can be detected from a variety of samples such as serum, feces, parasites, etc. Although studies have shown that they are sensitive and reliable, due to the limitations of equipment and PCR For reasons such as higher environmental requirements, these PCR techniques are limited to laboratories, so it is necessary to establish a sensitive, specific, and easy-to-use on-site diagnostic method

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