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44results about How to "Results visualization" patented technology

Aptamer-based rapid kanamycin detection test paper as well as preparation method and application thereof

The invention discloses aptamer-based rapid kanamycin detection test paper as well as a preparation method and application thereof, and belongs to the field of analytical chemistry as well as safety detection on medicine, environment and food. The aptamer-based rapid kanamycin detection test paper comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane, a water absorbing pad, a PVC bottom plate, a detection line and a quality control line. By the preparation method, by virtue of the characteristics that gold nanoparticles and silver nanoparticles are easy to prepare and modify and can dually amplify a signal as well as the conformational change of an aptamer, the strength change of an optical signal of the detection line is achieved, and thus detection on kanamycin is achieved; by using a test paper method for detecting the kanamycin, simple, convenient and fast detection is achieved, detection can be performed anytime and anywhere, a result is visualized, the detection time is short, an experimental result can be obtained within 10min by just inserting one end of the sample pad of the test paper into detection liquid, and the detection efficiency can be greatly improved; through a reading of a colloidal gold quantometer, quantitative analysis can be achieved; therefore, the aptamer-based rapid kanamycin detection test paper is of great significance in detection on kanamycin residue in animal-derived food.
Owner:JIANGNAN UNIV

Communication method and apparatus for wireless CPE (Customer Premise Equipment) and radio base station AP (Access Point) alignment

InactiveCN106879013AEasy to viewTroubleshoot poor wireless performanceWireless communicationSignal qualityBandwidth requirement
The invention discloses a communication method and apparatus for wireless CPE (Customer Premise Equipment) and radio base station AP (Access Point) alignment. The communication method includes the following steps that: CPE searches a radio base station AP (Access Point) and accesses a WLAN; the CPE transmits an alignment request to a radio base station AP (Access Point) which is connected with the CPE; the radio base station AP (Access Point) receives the alignment request and then executes alignment operation: a data traffic channel and a signal quality feedback channel are built between the CPE and the radio base station AP; wireless bandwidth obtained by the data traffic channel is compared with a preset wireless bandwidth lowest threshold value, and whether a bandwidth requirement is satisfied is judged, wireless signal strength obtained by the signal quality feedback channel is compared with a preset wireless signal minimum value, and whether a signal quality requirement is satisfied is judged; and when the bandwidth requirement and the signal quality requirement are both satisfied, alignment operation is completed, and whether an installation implementation process satisfies requirements is judged, if the installation implementation process do not satisfy any one of the requirements, the installation implementation process is not endorsed.
Owner:深圳云网通信科技有限公司

LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof

The invention discloses a LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof. The LAMP primer group is a primergroup 16SrDNA-2 or a primer group 16SrDNA-3. The LAMP primer group disclosed by the invention has very good specificity and stability, high detection sensitivity and the lowest detection limit of 200ag/[mu] L. Phytoplasma are detected in betel nut yellow leaf disease samples from Baoting, Tunchang, Wanning and the like in Hainan Province in China by using the two sets of LAMP primers, i.e., 16SrDNA-2 and 16SrDNA-3; and no phytoplasma is detected in negative control. The LAMP primer group and kit provided by the invention are rapid and efficient in detection, simple and convenient to operateand visual in result, play a great role in methods for early detection and field diagnosis of the betel nut yellow leaf diseases, bacteria-carrying detection of betel nut seedlings, breeding of resistant betel nut varieties and the like, and provide technical support and reference basis for pathogen detection, disease prevalence, scientific prevention and control and the like of the betel nut yellow leaf diseases.
Owner:COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI

Primer groups, kit and method for detecting specific sequence of glyphosate-resistant transgenic soybean transformant

The invention discloses primer groups, a kit and a method for detecting a specific sequence of a glyphosate-resistant transgenic soybean transformant. Two groups of LAMP (loop-mediated isothermal amplification) primers are designed, the primer group 1 is used for detecting an internal reference gene Lectin and can detect soybean ingredients in a sample and simultaneously exclude false negative results, and the primer group 2 is used for detecting the specific sequence of the glyphosate-resistant transgenic soybean transformant, has high specificity and can effectively detect glyphosate-resistant transgenic soybean ingredients, wherein the sequence is a boundary sequence which connects an inserted exogenous gene with a plant gene. When the kit is used for the detection and analysis of a sample, a positive control, a blank control and an internal control are respectively set, so that the production of false positives and false negatives can be avoided. Special equipment is not required for detection, and the detection cost is low; and the detection results can be judged by visual observation of changes in color of a reaction solution, the identification is simple and convenient to perform, the results are visualized, and the primer groups, the kit and the method disclosed by the invention are suitable for field fast detection and grassroots screening of a large number of samples and easy to realize large-range popularization and application.
Owner:GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION

Method and sensor for rapid visualization analysis of total amount of various phthalate pollutants

The invention belongs to the field of environmental engineering and water pollution detection and treatment, and relates to a nucleic acid aptamer colorimetric sensor and a preparation method and application thereof: a method for rapid visualization analysis of total amount of various phthalate pollutants based on the nucleic acid aptamer colorimetric sensor. A broad-spectrum nucleic acid aptamer with a specific base sequence and specific recognition ability for a class of structurally similar PAEs is combined with a highly sensitive colorimetric analysis technology to construct the nucleic acid aptamer colorimetric sensor for detecting the total amount of PAEs. According to the invention, the broad-spectrum nucleic acid aptamer capable of binding various PAEs with high affinity is used as a recognition component to successfully realizes high sensitivity, rapid and visualization detection analysis of the total amount of the substance; the linear detection range of PAEs is 0.003 mu g/L to 10 mu g/L, and the detection limit is as low as 1ng/L; and compared with the prior art, the sensor has the advantages of simple operation, fast response, easy miniaturization and detection result visualization.
Owner:TONGJI UNIV

Oceanographic engineering complex node multi-robot welding cooperative control method

The invention discloses an oceanographic engineering complex node multi-robot welding cooperative control method which comprises the following steps: Step 1, importing a model, and performing weld joint point selection and information extraction, Step 2, calculating a weld joint equation, Step 3, selecting one weld joint of a main welding robot, Step 4, selecting one weld joint of an auxiliary welding robot which works at the same time, Step 5, discretizing weld joint coordinates, Step 6, performing coordinate conversion on main and auxiliary weld joints under a global coordinate system, Step7, performing inverse motion calculation on the main and auxiliary weld joints to obtain joint corner data of the main and auxiliary welding robots, Step 8, repeating the Step 3 to the Step 7 until all calculation is completed, and Step 9, generating a welding robot motion instruction to realize cooperative welding of the welding robots. According to the method, the welding efficiency is improved,the welding cost is reduced, the operation process is simplified, the welding quality is improved, and the multi-robot welding cooperative control method is easy to operate, visual in result, stable,reliable and wide in adaptability.
Owner:BOMESC OFFSHORE ENG CO LTD

Auxiliary positioning device for apron board bracket of EMU and positioning method thereof

An auxiliary positioning device for an apron board bracket of EMU and a positioning method thereof belong to the technical field of rail vehicle apron board bracket positioning. The device comprises ahorizontal reference base, a bubble level, two horizontal slider devices, a T-shaped chute centerline positioning mechanism, a linear laser and a laser rangefinder, wherein the right side of the upper end surface of the horizontal reference base is provided with a base horizontal chute along the Y-axis direction, and the left side of the upper end surface of the horizontal reference base is provided with a base vertical through hole along the direction vertical to the Z axis; the two horizontal slider devices are slidingly connected to the base horizontal chute; both the linear laser and thelaser rangefinder are in sliding connection with the right side of the upper end surface of the horizontal reference base through a corresponding horizontal slider device in a vertical upward positionon the optical axis; and the middle section of the T-shaped chute centerline positioning mechanism is coaxially fixed with the base vertical through hole. The auxiliary positioning device for the apron board bracket of EMU and the positioning method thereof provided by the invention optimize the surveying and mapping process, enable the auxiliary positioning device of the apron board bracket to obtain the advantages such as accurate positioning, easy operation and visible results, and can greatly improve measurement accuracy and positioning efficiency.
Owner:CRRC CHANGCHUN RAILWAY VEHICLES CO LTD

Method for rapidly and quantitatively detecting enterobacter sakazakii based on cascade amplification

The invention constructs a biosensor for rapidly and quantitatively detecting enterobacter sakazakii based on cascade amplification. According to the sensor, quadruple cascade amplification of enterobacter sakazakii is realized through magnetic enrichment, antibody-nucleic acid aptamer recognition, EXPAR and DNAzyme signal accumulation. A method comprises steps: firstly, immunomagnetic beads are prepared for magnetic enrichment; secondly, a target is directly captured from a complex matrix through magnetic separation, then a nucleic acid aptamer is added, an immune sandwich structure of an antibody-target-fusion nucleic acid aptamer is formed, and high specificity of the sensor is guaranteed; thirdly, exponential amplification of the signal is realized through EXPAR; and finally, a quadruplex with enzyme-like activity is formed through a G-rich sequence in the product, and color development signal output is catalyzed. The method has the advantages of high sensitivity, high specificity, rapidness, simplicity and convenience, the quantitative detection of enterobacter sakazakii can be realized within 2 hours, the whole process is extraction-free, the result is visual, and the problems of complex sensor preparation and long determination time are effectively solved.
Owner:CHINA AGRI UNIV

Information management method for oil and gas module process pipeline welding seam

The invention discloses an information management method for an oil and gas module process pipeline welding seam. The information management method comprises the steps of establishing a welding seam information extraction platform based on TEKLA software, and establishing a welding seam information database and a welding seam information data table of an oil and gas module process pipeline; formulating a coding rule to generate a welding seam bar code comprising a welding seam number, welding seam attribute information and welding seam welding process information, and associating the welding process information and the welding seam detection process information of each welding seam with the welding seam bar code by using a VB # language. By scanning the welding seam bar code at the welding seam, welding length information, welding height information, welding leg size information, welding material information, base material information, welding type information, welding process flow information and welding seam detection process flow information of the welding seam can be checked, and information management of the oil and gas module process pipeline welding seam is achieved. The method effectively solves the problems that in a traditional pipeline welding seam information management method, the welding seam needs to be operated on site, the efficiency is low, and the cost is high; and the method is efficient and reliable.
Owner:TIANJIN UNIV

A monitoring method for the maximum deformation during the lifting process of large structures

The invention discloses a method for monitoring the maximum amount of deformation during the lifting process of a large-scale structure, which includes irradiating the surface of the structure with laser light to form laser speckles, and using a CCD camera to take pictures at a certain time interval when it is not lifted and during the lifting process. The captured grayscale image is processed by the image acquisition card to obtain the pixel coordinates of the speckle, and the K-means module in Python is used to cluster the coordinate data, and a cluster center point is selected in each speckle as the scatter point. The approximate replacement of the spot position, calculate the offset of these corresponding pixels in matlab, obtain the maximum offset, and draw the line graph of the offset over time. During the promotion process, if there is a special situation in the line graph, you need to immediately Shut down for maintenance. The invention can improve the measurement accuracy of the surface deformation of the structure, simplify the setting process of the measurement device, reduce the use cost and greatly improve the measurement efficiency.
Owner:BOMESC OFFSHORE ENG CO LTD

Method for automatically calculating counter weight in circular track type crane

The invention discloses a method for automatically calculating a counter weight in a circular track type crane, which comprises the following steps that codes of a three-dimensional model of the circular track type crane are written by using an APDL language, so that the three-dimensional model of the circular track type crane established in AutoCAD software is smoothly imported into SAP2000 software, and finite element analysis is performed on the three-dimensional model of the circular track type crane. The three-dimensional model of the circular track type crane is initialized and loaded by adopting simulation setting in SAP2000 software, and the bearing reaction of each hinge point at the counterweight end in the three-dimensional model of the circular track type crane is calculated through a finite element algorithm, so that the weight required by the counterweight of the circular track type crane is calculated. According to the method, the problems that the counter weight calculation process of the circular track type crane is complex and the accuracy is not high are effectively solved, the finite element analysis technology of structural analysis and design software is utilized, and the actual counter weight of the circular track type crane is obtained by carrying out practical configuration on a three-dimensional model of the circular track type crane through a computer; and the safe and reliable operation of the circular track type crane is ensured.
Owner:TIANJIN UNIV
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