RT-LAMP method for identifying Japanese encephalitis virus gene types I and III

A RT-LAMP, Japanese encephalitis virus technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of long time, complicated operation, high cost, and achieve easy identification, visualization of results, and timeliness. The effect of sexual dominance
CN104388591AInactive Publication Date: 2015-03-04SICHUAN AGRI UNIV
3 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2015-03-04
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an RT-LAMP method for identifying Japanese encephalitis virus gene types I and III. Through the comparative analysis of Japanese encephalitis virus gene nucleotide sequences of gene types I and III, four specific primers for specific amplification of Japanese encephalitis virus nucleic acids of gene types I and III are designed for six regions on a target gene, the reaction result can be determined according to the color change of liquid in a reaction tube when a reaction is finished after calcein fluorochrome is added to a reaction system, and the reaction result can be determined according to whether a precipitate exists in the reaction tube or not after the reaction is finished or whether a ladder band appears or not during electrophoresis. According to the method, under the constant temperature condition, reverse transcription and nucleic acid amplification are realized within 1h, and the Japanese encephalitis virus gene types I and III can be quickly detected and identified within 1h.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to an RT-LAMP method for identifying Japanese Japanese encephalitis virus genotype I and genotype III, specifically, using reverse transcription-loop-mediated isothermal amplification technology to identify genotype I and genotype III Japan Japanese encephalitis virus can be used to distinguish between disease diagnosis purposes and non-disease diagnosis purposes. Background technique

[0002] Japanese encephalitis B (JE), also known as epidemic encephalitis (epidemic encephalitis B), is a mosquito-borne infectious disease caused by Japanese encephalitis virus (JEV) , JEV is the main causative agent of viral encephalitis in Asia, which is a flavivirus borne by mosquitoes. The population of the endemic area is as high as 3 billion, and at least 50,000 people are reported to be infected with Japanese encephalitis virus every year. Japanese encephalitis virus is widespread from Asia to northern Australia. With the application of m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More