The invention provides a Chinese medicine preparation capable of diminishing inflammation, relieving pains, resisting bacteria, expelling stone and benefiting gallbladder, a preparation method thereof and applications of the Chinese medicine preparation in preparing medicines for treating urinary system calculi, urinary tract infection, cholecystitis, gall-stone and prostatitis. According to the theory of Chinese medicines, through the experiment and screening for many years, the invention reselects raw drugs in the prescription, optimizes the preparation technology, greatly enhances the medicinal functions of promoting diuresis for stranguria and removing heat for detumescence, has unexpected obvious curative effect on treating urinary system diseases, and especially produces unexpected effects of resisting bacteria, resisting prostatitis, relieving pains and benefiting gallbladder which are not provided in the prior art. The Chinese medicine preparation of the invention exerts the traditional advantages of Chinese medicines, takes effect quickly, obviously improves the subjective symptom and the physical sign, and does not have obvious side effect.