LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof

A primer set and phytoplasma technology, applied in the field of identification and control, plant disease detection, can solve the problems of specificity, sensitivity difference, etc.

Inactive Publication Date: 2020-05-01
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
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Problems solved by technology

[0006] Phytoplasma housekeeping genes of different groups and different hosts, such as 16S rRNA, tuf, groEL and other ge

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  • LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof
  • LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof
  • LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, it should be understood that the described embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solution of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications or replacements all fall within the protection scope of the present invention.

[0028] 1 Materials and methods

[0029] 1.1 Materials

[0030] 1.1.1 Experimental materials

[0031] The betel nut yellowing samples were collected from Baoting, Tunchang and Wanning, Hainan in July 2019, and the healthy betel nut samples were collected from Wanning, Hainan in July 2019.

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Abstract

The invention discloses a LAMP primer group and kit for detecting 16SrI-group phytoplasma leading to betel nut yellow leaf diseases in China, and application thereof. The LAMP primer group is a primergroup 16SrDNA-2 or a primer group 16SrDNA-3. The LAMP primer group disclosed by the invention has very good specificity and stability, high detection sensitivity and the lowest detection limit of 200ag/[mu] L. Phytoplasma are detected in betel nut yellow leaf disease samples from Baoting, Tunchang, Wanning and the like in Hainan Province in China by using the two sets of LAMP primers, i.e., 16SrDNA-2 and 16SrDNA-3; and no phytoplasma is detected in negative control. The LAMP primer group and kit provided by the invention are rapid and efficient in detection, simple and convenient to operateand visual in result, play a great role in methods for early detection and field diagnosis of the betel nut yellow leaf diseases, bacteria-carrying detection of betel nut seedlings, breeding of resistant betel nut varieties and the like, and provide technical support and reference basis for pathogen detection, disease prevalence, scientific prevention and control and the like of the betel nut yellow leaf diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease detection, identification and prevention, and relates to a primer for detecting phytoplasma, in particular to a LAMP primer set, kit and application for detecting 16SrI group areca phytoplasma phytoplasma in my country. Background technique [0002] Phytoplasma (phytoplasma) is a kind of prokaryotic pathogenic bacteria that parasitize in the phloem of plants, has no cell wall, and cannot be isolated and cultured artificially. Its host range is wide and the damage is serious. There are more than 1,000 species of plants affected worldwide, and more than 100 species of phytoplasma diseases have been reported in my country, causing huge losses to economic crops and green plants in our country. Betel nut (Areca cathecu L.) is a tropical evergreen plant of the palm family and one of the important resources of southern medicine in my country. Betel nut yellowing is a devastating disease in betel n...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6844C12N15/11
CPCC12Q1/6844C12Q1/6895C12Q2531/119
Inventor 于少帅覃伟权阎伟宋薇薇唐庆华覃一凌
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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