Porcine circovirus type 2 LAMP detection primer group and kit

Abstract

The invention discloses a porcine circovirus type 2 LAMP (Loop-Mediated Isothermal Amplification) detection primer group and a kit, the porcine circovirus type 2 LAMP detection primer group comprises six primers, and the specific nucleotide sequences of the six primers are respectively as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. The porcine circovirus type 2 LAMP detection kit comprises the primer group, and also comprises the following components: Bst DNA polymerase, dNTP, MgSO4, Betaine, a 10X LAMP reaction buffer solution, HNB, a positive control plasmid and the like. The kit disclosed by the invention has a series of advantages of high sensitivity, good specificity, short reaction time, result visualization, simple matched instruments and the like, and is not only suitable for large breeding units and high-end laboratories, but also suitable for vast small and medium breeding units and common laboratories.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and specifically, the porcine ring virus type 2 LAMP detection primer group and kit. Background technique [0002] Porcine Circovirus (PCV) belongs to the circoviridae ring virus, which is a single-stranded DNA virus, a phenocapstick. Depending on its discovery, the difference in pathogenic and nucleic acid sequences can be divided into type 2, ie PCV1 and PCV2. PCV1 is not pathogenic, while PCV2 has a pathogenic. [0003] Porcine Circovirus Type 2 (PCV2) is a DNA virus with a single negative chain cyclic phenademic membrane, and its genome is 1.7KB. The PCV2 infection mainly causes the POSTWEANING MULTISISTEMIC WANDROME (PMWS), which can be combined with many pathogens, causing immunosuppression in pigs. [0004] Since the first outbreak of PCV2 in the Canadian pigs in 1991, it is widely popular among the pigs around the world. PCV2 Relevance Disease, PCVAD is a complex, multi-factor disease, in ...

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Problems solved by technology

[0005] Diagnosis methods for PCV2 include clinical diagnosis, virus isolation and identification, enzyme-linked immunosorbent assay (ELISA), common PCR and fluorescent quantitative PCR, etc. Among them: clinical diagnosis cannot be confirmed, virus isolation and identification are too complicated and cumbersome, and ELISA detection can only be performed in animals. Antibodies can only be detected later, which leads to a delay in detection time. Ordinary PCR detection requires electrophoresis after amplification to obtain detection results, while fluorescent quantitative PCR detection requires the use of expensive fluorescent quantitative PCR instruments, which limits the scope of use of this method.

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