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Phase Contrast Imaging with Transfer Function

A transfer function, optical imaging technology, applied in the field of optical systems, which can solve the problems of imaging frequency amplification, restricting the use of QDPC, etc.

Active Publication Date: 2022-04-15
CARL ZEISS MICROSCOPY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This may limit the ability to use QDPC in practical problems
Furthermore, it can be observed that, for example, an undesired amplification of the imaging frequency can occur in QDPC if there is a certain tolerance in the adopted aperture

Method used

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  • Phase Contrast Imaging with Transfer Function
  • Phase Contrast Imaging with Transfer Function
  • Phase Contrast Imaging with Transfer Function

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Experimental program
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Embodiment Construction

[0027] Through the description of the following exemplary embodiments, the above-mentioned characteristics, characteristics and advantages of the present invention and the manner of realizing these characteristics and advantages will become clearer and more clearly understandable, and these exemplary embodiments will be further described through the accompanying drawings Detailed explanation.

[0028] In the drawings, the same reference numerals designate the same or similar elements. The figures are schematic illustrations of different embodiments of the invention. Elements shown in the drawings are not necessarily drawn to scale. Rather, the various elements shown in the figures are depicted in such a way that those skilled in the art can understand their function and generality. The connections and couplings between functional units and elements shown in the figures can also be implemented as indirect connections or couplings. Connection or coupling can be accomplished i...

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PUM

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Abstract

An optical system (100) comprising: a sample holder (113) configured to hold a sample object; an illumination module (111) configured to illuminate the sample object with at least one structured illumination geometry (300); an optical an imaging unit (112) configured to generate an image representation of said sample object illuminated by at least one structured illumination geometry (300) on a detector (114); a detector (114) configured to At least one image of the sample object is captured based on the image representation; a controller (115) configured to determine a resulting image with phase contrast based on the transfer function (400) and the at least one image. Here, said transfer function (400) corresponds to a reference transfer function (400) that has been scaled based on the aperture size of the optical imaging unit (112).

Description

technical field [0001] Various examples of the invention generally relate to an optical system having an illumination module configured to illuminate a sample object with a structured illumination geometry. Various examples of the invention relate inter alia to techniques for determining a resultant image with phase contrast based on a transfer function and at least one image of a sample object. Background technique [0002] In optical imaging of sample objects it is often of value to generate so-called phase-contrast images of the sample object. In a phase contrast image, at least part of the image contrast is caused by the phase shift of light passing through the sample object being imaged. In particular, this enables sample objects that cause no attenuation, or only a small attenuation of the amplitude, but a significant phase shift, to be imaged with higher contrast. Often, such sample objects are also called phase objects. Biological samples, as sample objects under ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06G02B21/14G02B21/36
CPCG02B21/06G02B21/14G02B21/365G02B21/367
Inventor 拉尔斯·施托佩托马斯·米尔德
Owner CARL ZEISS MICROSCOPY GMBH