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Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker, primer pair, kit and application thereof

A technology of molecular markers and Yanbian Yellow Cattle, which is applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems that ANGPTL4 gene polymorphism research has not been reported.

Active Publication Date: 2019-12-06
YANBIAN UNIV
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AI Technical Summary

Problems solved by technology

From the above research progress, it can be seen that the ANGPTL4 gene has been widely studied in cattle, and there are many polymorphic sites. Different sites have different effects on traits, and the effects are also different in different breeds. The Yanbian yellow cattle ANGPTL4 gene Polymorphism studies have not been reported

Method used

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  • Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker, primer pair, kit and application thereof
  • Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker, primer pair, kit and application thereof
  • Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker, primer pair, kit and application thereof

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Experimental program
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Effect test

Embodiment 1

[0057] 1. Sample collection

[0058] The test cattle group selected 70 Yanbian yellow cattle bulls from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd., all the test cattle were fattened and slaughtered at the age of 30 months according to the unified standard feeding conditions, and slaughtered and divided according to the national unified standard. Add anticoagulant, aliquot and store at -80°C for later use.

[0059] 2. Extract DNA samples from Yanbian Yellow Cattle according to the instructions of the TIANGEN-Blood Genomic DNA Extraction Kit. The purity of the extracted Yanbian Yellow Cattle DNA sample was detected by ultraviolet spectrophotometer, and the OD 260 / OD 280 value. Agarose gel electrophoresis detection: use 1% agarose gel to detect the Yanbian Yellow Cattle DNA sample, and perform voltage electrophoresis for 30 minutes according to the fragment length; observe with a gel imaging system.

[0060] The results of the ultraviolet spectrophot...

Embodiment 2

[0088] 1. Experimental animals and sample collection

[0089] In this test cattle group, 70 Yanbian yellow cattle bulls from Jilin Province Yanbian Animal Husbandry Development Group Co., Ltd. were selected. All the test cattle were fattened and slaughtered at the age of 30 months according to the unified standard feeding conditions, and slaughtered and divided according to the national unified standard. , vacuum-packed and stored at low temperature for the determination of meat quality traits.

[0090] 2. Determination of main traits of cattle

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Abstract

The invention provides a Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker, a primer pair, a kit and an application thereof, and belongs to the technical field of cattle meatquality screening. The Yanbian yellow cattle meat quality-related ANGPT4 gene SNP molecular marker contains a nucleotide sequence in which the polymorphism of 41995bp of a gene is C / T. The polymorphism of the ANGPT4 gene and the meat quality trait are subjected to correlation analysis; the 41995bp C / T site has the significant correlation with the characters of the fat content, the protein content, the carcass weight and the marble pattern grade of Yanbian yellow cattle; and it is showed that the fat content, the carcass weight and the marble pattern grade of a CT genotype are obviously higherthan those of a CC genotype. The protein content of the CC genotype is significantly higher than that of the CT genotype. The SNP molecular marker of the ANGPT4 gene can be used as a meat quality trait screening-related gene marker and is used for high-grade beef cattle early-stage selection of the Yanbian yellow cattle.

Description

technical field [0001] The invention belongs to the technical field of yellow beef quality screening, and in particular relates to a Yanbian yellow beef quality-related ANGPTL4 gene SNP molecular marker, primer pair and application thereof. Background technique [0002] Along with the continuous development of economy and agriculture, while people's quality of life is improved, people's dietary structure is also gradually improved. Among the meats, beef has high nutritional value, and the consumption proportion of beef is increasing the fastest, so it is an ideal meat food. People's demand for high-grade beef is increasing day by day. Yanbian Yellow Cattle is mainly produced in Yanbian, Jilin Province, my country. It has the characteristics of the Yanbian Korean nationality. It is one of the five local fine breed cattle and is a high-quality local meat breed in my country. Yanbian yellow cattle have adapted to the local natural environment for many years. They are strong a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 夏广军徐红艳徐畅尹宝珍张洛萌
Owner YANBIAN UNIV
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