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Separation substrate, cell separation filter and platelet producing method

A technology of platelets and substrates, applied in the direction of sterilization methods, chemical instruments and methods, methods of supporting/immobilizing microorganisms, etc., can solve problems such as difficulty in maintaining the balance between demand and supply

Inactive Publication Date: 2019-12-24
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current platelet preparation depends on blood donation from volunteers. Although the effective period of storage is extremely short of 4 days, as the population of the age group who can donate blood due to declining birth rate decreases and the population of elderly people with high blood donation needs increases, Predicting the balance of demand and supply in the medical field is difficult to maintain

Method used

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  • Separation substrate, cell separation filter and platelet producing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136]

[0137] 15 parts by mass of polysulfone (P3500, manufactured by Amoco), 15 parts by mass of polyvinylpyrrolidone, 2 parts by mass of lithium chloride, and 1.2 parts by mass of water were dissolved in 66.8 parts by mass of N-methyl-2-pyrrolidone to obtain the prepared Membrane mixture.

[0138] The mixture was cast on the surface of a PET film to a thickness of 200 μm.

[0139] Next, air adjusted to 25° C. and an absolute humidity of 7.8 g / kg Air was applied to the surface of the cast liquid film at 2 m / sec for 5 seconds.

[0140] Thereafter, it was directly immersed in a coagulation tank filled with water at a temperature of 40°C.

[0141] Next, after PET was peeled off, it was placed in a diethylene glycol bath at 25° C. for 120 seconds at 2 m / sec, and then sufficiently washed with pure water to produce a porous membrane.

[0142]

[0143] Medium: 50 ml of bovine serum (Life Technologies) was added to 450 ml of RPMI1640 (Life Technologies).

[0144] Megakaryocy...

Embodiment 2 and comparative example 1～3

[0162] When making a porous film, change the moisture concentration contained in the temperature-regulated wet air, the time of applying the temperature-regulated wet air, and make a porous film showing the average pore diameter and thickness shown in Table 1 below. A separation base material was produced and evaluated in the same manner as in Example 1. The results are shown in Table 1.

Embodiment 3

[0164] Evaluation was performed by the same method as in Example 1 using a porous membrane made of hydrophilic polyvinylidene fluoride (SVLP04700, manufactured by Merck KGaA, Darmstadt, Germany). The results are shown in Table 1.

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Abstract

The present invention addresses the problem of providing: a separation substrate which has a high inhibition rate of megakaryocytes and a high permeability of platelets; a cell separation filter usingthe same; and a platelet producing method. The separation substrate of the present invention is composed of a porous membrane for separating platelets from cell suspension including megakaryocytes and platelets, wherein the average pore diameter of the separation substrate is 2.0-12.0 [mu]m, and the separation substrate is composed of at least one resin selected from the group consisting of a polysulfone resin and a polyvinylidene fluoride resin.

Description

technical field [0001] The invention relates to a method for manufacturing a separation substrate, a cell separation filter and platelets. Background technique [0002] Platelets are cells that play a central role in the formation of thrombus and exhibit a hemostatic function in vivo. Therefore, when platelets decrease during bleeding or when anticancer agents are used, it may lead to death in severe cases. [0003] Furthermore, the only established therapy for the reduction of platelets is the transfusion of platelet preparations. The current platelet preparation depends on blood donation from volunteers. Although the preservation period is extremely short of 4 days, as the population of the age group who can donate blood due to declining birth rate decreases and the population of elderly people with high need for blood donation increases, Predicting the balance of demand and supply in the medical field is difficult to maintain. [0004] Therefore, the development of a so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06A61K35/19A61P7/04B01D61/14B01D61/18B01D69/00B01D71/34B01D71/68C12M1/12C12N5/078
CPCA61P7/04B01D71/34B01D71/68C12M47/02A61K35/00C12N5/0644B01D61/147B01D69/02B01D2325/36A61K35/19B01D67/0013B01D2325/02C12M29/04
Inventor 武井俊树山田忠范竹上竜太神长邦行
Owner FUJIFILM CORP
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