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A nanoemulsion loaded with antigen based on mf59 and its preparation method and application

A technology of nanoemulsion and antigen, which is applied in the direction of antineoplastic drugs, pharmaceutical formulations, emulsion delivery, etc., to achieve good curative effect, reduce storage costs, and enhance the effect of nanoemulsion adjuvant

Active Publication Date: 2021-07-30
SHANGHAI DERMATOLOGY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A nanoemulsion loaded with antigen based on mf59 and its preparation method and application
  • A nanoemulsion loaded with antigen based on mf59 and its preparation method and application
  • A nanoemulsion loaded with antigen based on mf59 and its preparation method and application

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Effect test

Embodiment 1

[0042] Embodiment 1 component (one)

[0043] Take each component according to the following proportions by weight:

[0044] 2.58 parts of squalene, 50.9 parts of emulsifier span8, 00.9 parts of tween, 20 parts of PBS, 0.05 parts of chicken ovalbumin.

Embodiment 2

[0045] Embodiment 2 components (two)

[0046] Take each component according to the following proportions by weight:

[0047] 2 parts of squalene, 1 part of emulsifier span851, 1 part of tween801, 15 parts of PBS, 0.1 part of chicken ovalbumin.

Embodiment 3

[0048] Embodiment 3 Vaccine (1)

[0049] The preparation method is as follows:

[0050] (1) Take 2.58 parts of squalene, 50.9 parts of emulsifier span850.9 parts and 0.6 parts of tween800.6 parts, and mix them to make an oil phase;

[0051] (2) Dissolve 0.05 part of chicken ovalbumin in 0.3 part of pure water to make the inner water phase;

[0052] (3) Dissolve 0.3 parts of tween80 in 20 parts of PBS solution to make the outer water phase;

[0053] (4) Add the inner water phase prepared in step (2) to the oil phase prepared in step (1) to form reverse micelles, then add the reverse micelles to the outer water phase prepared in step (3) A double emulsion is formed in the phase.

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Abstract

The invention belongs to the technical field of pharmaceutical preparations, and specifically relates to a MF59-based antigen-encapsulated nanoemulsion and its preparation method and application. The weight ratio of its components is: 2.58 parts of squalene, 0.9 parts of emulsifier span85, and 0.9 parts of tween80 , 20 copies of PBS, 0.005-0.05 copies of antigen. Antigen-loaded nanoemulsions were prepared by double-emulsion method based on reverse micelles, including the following steps: firstly adding the inner water phase to the oil phase containing emulsifier to form reverse micelles, and then adding the reverse micelles to the outer A double emulsion is formed in the aqueous phase. The present invention also includes the application of the MF59-based antigen-carrying nanoemulsion in vaccines. Its advantages are as follows: improving the encapsulation efficiency of macromolecular proteins carried by nanoemulsions, while controlling the particle size within 100nm.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to a MF59-based antigen-encapsulated nanoemulsion and its preparation method and application. Background technique [0002] Nanoemulsion is composed of oil phase, water phase, emulsifier and co-emulsifier, etc. It is a colloidal dispersion system formed by dispersing emulsion droplets with a particle size of 10-100nm in another solution. Nanoemulsions can not only entrap fat-soluble drugs, improve their solubility, and promote oral absorption and transdermal transport of drugs; they can also entrap water-soluble drugs, especially macromolecular protein drugs, to improve the oral bioavailability and economical efficiency of macromolecular drugs. Skin transfer. In addition, studies have found that oil-in-water nanoemulsions can also be used as immune adjuvants to enhance the immune effect of antigens. MF59 is an oil-in-water emulsion that can enhance the immunoge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K39/39A61K47/44A61K47/26A61K47/14A61K47/06A61P35/00
CPCA61K9/1075A61K39/39A61K47/06A61K47/14A61K47/26A61K47/44A61K2039/55566A61P35/00Y02A50/30
Inventor 喻琴谢欣辛武喜营朱全刚连天雁李莹沈敏
Owner SHANGHAI DERMATOLOGY HOSPITAL
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