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Flexible Guided Piezoelectric Drilling Device with Large Axial Vibration and Small Lateral Vibration

A technology of lateral vibration and axial vibration, applied in biochemical cleaning equipment, enzymology/microbiology equipment, laboratory equipment, etc., can solve problems such as oocyte deformation

Active Publication Date: 2022-04-05
苏州博致医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this design still induces such a considerable lateral vibration of 20 μm and results in deformation of the oocyte larger than 10 μm

Method used

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  • Flexible Guided Piezoelectric Drilling Device with Large Axial Vibration and Small Lateral Vibration
  • Flexible Guided Piezoelectric Drilling Device with Large Axial Vibration and Small Lateral Vibration
  • Flexible Guided Piezoelectric Drilling Device with Large Axial Vibration and Small Lateral Vibration

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0054] Material:

[0055] A scanning electron microscope (SEM, Hitachi SU3500) was used to characterize the axial and lateral vibration amplitudes of the micropipettes. A laser Doppler vibrometer (OFV-5000, Polytec) was used to verify the axial vibration amplitude of the micropipette measured by SEM. The displacement resolution of the vibrating meter can reach 1pm.

[0056] result:

[0057] SEM imaging has a low bandwidth (20 Hz); therefore, when a micropipette vibrates, its vibrational envelope appears as a fuzzy edge in the SEM image [see Figure 6 (b)(C)]. Measuring the distance between the edge of the micropipette and its corresponding vibration-induced blurred edge enabled the quantification of the axial and lateral vibration amplitude of the micropipette.

[0058] Figure 6 (b) and (c) respectively correspond to 15 kHz and 18 kHz, which are the frequencies of the driving pulses supplied to the piezoelectric actuator. The peak voltage of the driving pulse was kept c...

example 2

[0060] Material:

[0061] Mouse oocytes were collected from the Canadian Mouse Mutant Repository at the Toronto Center for Phenogenomics. Oocytes were observed using an inverted microscope (Nikon Ti, Nikon Microscopes) and a CCD camera (acA1300-30gm, Basler).

[0062] result:

[0063] Based on the test on 45 mouse oocytes, the ZP penetration success rate using the piezoelectric drill device of the present invention is 100%. Figure 8 (a)(b), corresponding to a supplied driving pulse of 18 kHz and a peak voltage of 20 V, showing that the piezoelectric drill device is able to penetrate the ZP of a mouse oocyte with deformation as small as 3.4 μm. In contrast, existing piezoelectric drill devices produce mouse oocyte deformations greater than 10 μm unless damped with a drop of mercury in the micropipette.

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Abstract

The present invention describes a flexible guided piezoelectric drill device that produces large axial vibrations and small lateral vibrations. A flexible mechanism is used to guide the trajectory of the micropipette in the axial direction and to remove the resonant frequencies that cause large transverse vibrations from the drive pulse. The invention is particularly suitable for efficient penetration of the zona pellucida (ZP) of oocytes and embryos with little deformation.

Description

technical field [0001] The present invention relates to an inertial impact injection device, and more particularly to its use in the manipulation of oocytes and embryos for in vitro fertilization. Background technique [0002] Penetration of the zona pellucida (ZP) is an important step in in vitro fertilization (IVF), with applications such as intracytoplasmic sperm injection (ICSI) and biopsy for preimplantation genetic screening (PGS). The ZP is a thick, highly elastic layer composed of glycoproteins surrounding the oocyte or embryo. [0003] In oocyte and embryo manipulations in IVF clinics, ZP penetration requires severe deformation of the oocyte or embryo (eg, 50 μm), even with a sharp micropipette. Large oocyte deformations disrupt the oocyte spindle and lead to failure of oocyte fertilization and embryonic development. Large embryo deformations can also affect subsequent embryonic development. Vibration using piezoelectric actuation can improve ZP penetration witho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M1/00C12M21/06C12M33/04B01L3/022B01L2400/0439
Inventor 孙钰韦斯利·约翰逊刘军戴昌盛
Owner 苏州博致医疗科技有限公司