Molecular marking method for predicting and identifying chicken abdominal fat amount and applications thereof

A molecular marker, abdominal fat technology, applied in the field of animal molecular genetics, can solve the problems that have not yet been discovered, and achieve the effect of high accuracy, low cost, and speeding up the breeding process

Active Publication Date: 2020-03-24
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the selection of chicken abdominal fat content by HBP1 gene molecular markers

Method used

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  • Molecular marking method for predicting and identifying chicken abdominal fat amount and applications thereof
  • Molecular marking method for predicting and identifying chicken abdominal fat amount and applications thereof
  • Molecular marking method for predicting and identifying chicken abdominal fat amount and applications thereof

Examples

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Effect test

Embodiment 1

[0036] Example 1. Molecular marker method for predicting and identifying chicken belly fat mass.

[0037] 1. Design and synthesis of primers.

[0038] Primers were designed according to the SNP site g.-4354T>A upstream 337bp downstream 27bp sequence of the chicken HBP1 gene, and were synthesized by Jinweizhi Biotechnology Co., Ltd. The primer sequences are as follows:

[0039] HBP1-F:5'-TGTTAATTGCTAAGGGTGGA-3'

[0040] HBP1-R:5'-AGCACAAATAACCAAGAATTACTCG-3'

[0041] 2. Using the amplification primers obtained in step 1 to perform PCR amplification of chicken genomic DNA to obtain amplification products.

[0042] DNA extraction, product amplification:

[0043] 1) Extraction of chicken DNA, chicken DNA small sample extraction can adopt the following two methods:

[0044] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 100-200 μg / ml, mix and digest at 55°C for 12 hours until there are no sticky lumps...

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Abstract

The invention discloses a molecular marking method for predicting and identifying chicken abdominal fat amount and applications, and belongs to the technical field of animal molecular genetics. The molecular marking method for predicting and identifying chicken abdominal fat amount can identify low-fat broilers more accurately and conveniently, and accelerate broiler breeding process. According tothe method, primers are designed according to chicken HBP1 gene SNP site g. -4354T>A upstream 337bp and downstream 27bp sequence; and PCR amplification on chicken genome DNA is carried out accordingto the obtained primers, then forced enzyme digestion on the amplification product by using restriction enzymes is carried out, and polyacrylamide gel electrophoresis separation on enzyme digestion products is carried out to obtain the chicken abdominal fat labeled genotypes. The method is simple to operate, low in cost and high in accuracy, can be used for automatic detection, is an effective molecular marker breeding means, can provide a scientific basis for marker-assisted selection of chickens, and can be used for accelerating the breeding process of the chickens and breeding low-fat broilers meeting market requirements, so that relatively good economic benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of animal molecular genetics, and in particular relates to a molecular marker method and application for predicting and identifying chicken abdominal fat mass. Background technique [0002] After long-term selective breeding, the growth rate and meat yield of broilers have been significantly improved, but broiler breeding is facing new challenges. Accompanied by rapid growth, physiological discomforts and related diseases of broiler chickens have increased significantly, such as excessive body fat accumulation, ascites syndrome, sudden death syndrome, leg diseases, and decreased immune function of the body. These problems have caused huge harm to broiler chicken producers. Economic losses. Excessive accumulation of body fat (especially abdominal fat) in broilers has become a prominent problem. Excessive fat deposition in broilers has many disadvantages: (1) significantly reduces feed conversion efficiency,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王守志张长超
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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