A method for preparing s-3-dimethylamino-1-(2-thienyl)-1-propanol by biocatalysis

A technology of dimethylamino and S-3-, which is applied in the field of biocatalytic preparation of S-3-dimethylamino-1--1-propanol, can solve the problems of increasing operation difficulty and cost, and achieve the goal of preparing The process is green and environmentally friendly, with high specific enzyme activity and low discharge of three wastes
CN110923277BActive Publication Date: 2021-05-11NANJING LANGEN BIOLOGICAL SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING LANGEN BIOLOGICAL SCI & TECH
Publication Date
2021-05-11

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Abstract

The invention discloses a biocatalytic method for preparing S-3-dimethylamino-1-(2-thienyl)-1-propanol, which belongs to the field of biotechnology. The 3-dimethylamino-1-( 2-thienyl)-1-acetone hydrochloride is converted into S-3-dimethylamino-1-(2-thienyl)-1-propanol, which has higher alcohol dehydrogenase activity and has K49R, One or more mutations of A68T, E101D, F147E, T152A, S169V, A235S. The entire system of the present invention is catalyzed by a single enzyme, using alcohols for the coenzyme cycle, the substrate concentration is as high as 100g / L-160g / L, the chiral purity is greater than 99%, the amount of substrate / coenzyme is as high as 6000:1, and the number of cycles of coenzyme High, making S-3-dimethylamino-1-(2-thienyl)-1-propanol production cost effectively reduced.
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Description

technical field

[0001] The invention relates to a method for preparing S-3-dimethylamino-1-(2-thienyl)-1-propanol by biocatalysis, and belongs to the field of biotechnology. Background technique

[0002] Ketoreductases are versatile catalysts through the enantioselective reduction of aldehydes or ketones to the corresponding alcohols; (R)-specific ketoreductases have distinct properties from (S)-specific ketoreductases, and these catalysts are Optically active alcohols are used more and more frequently in the industrial synthesis; optical activity is a necessary condition for the selective action of many pharmaceutically and agrochemically active compounds, and in some cases one enantiomer has beneficial pharmaceutical However, enantiomers have genotoxic effects; therefore, in the synthesis of pharmaceutical and agricultural active compounds, it is necessary to use catalysts with the required stereospecificity to synthesize optically active alcohols.

[0003] Duloxetine is ...

Claims

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