A kind of activity enhanced ketoreductase mutant and its application

An activity-enhancing, reductase technology, applied in the directions of oxidoreductase, application, enzyme, etc., can solve the problems of increasing the difficulty of operation and cost, and achieve the effect of high cycle times of coenzymes, low three-waste emissions, and high specific enzyme activity
CN110938609BActive Publication Date: 2021-07-23NANJING LANGEN BIOLOGICAL SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING LANGEN BIOLOGICAL SCI & TECH
Publication Date
2021-07-23

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Abstract

The invention discloses a ketoreductase mutant with enhanced activity and its application, which belongs to the field of biotechnology. It is derived from the wild-type ketoreductase of Lactobacillus parabuchneri, which shows that the substrate (such as 3-dimethylamino-1 -(2-thienyl)-1-acetone hydrochloride) to products such as S-3-dimethylamino-1-(2-thienyl)-1-propanol) with increased conversion rates of ketoreductases. Compared with the wild-type ketoreductase of SEQ ID NO.7, the mutant ketoreductase exhibits stronger catalytic activity. The mutants of the present invention can be obtained by in vitro recombination, polynucleotide mutagenesis, DNA shuffling, error-prone PCR and directed evolution methods that encode the enzyme.
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Description

technical field

[0001] The invention relates to an enzyme, a biocatalysis method and its application, in particular to an activity-enhanced ketoreductase mutant and its application, and belongs to the field of biotechnology. Background technique

[0002] Ketoreductases are versatile catalysts through the enantioselective reduction of aldehydes or ketones to the corresponding alcohols; (R)-specific ketoreductases have distinct properties from (S)-specific ketoreductases, and these catalysts are Optically active alcohols are used more and more frequently in the industrial synthesis; optical activity is a necessary condition for the selective action of many pharmaceutically and agrochemically active compounds, and in some cases one enantiomer has beneficial pharmaceutical However, enantiomers have genotoxic effects; therefore, in the synthesis of pharmaceutical and agricultural active compounds, it is necessary to use catalysts with the required stereospecificity to synthesize ...

Claims

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