Treponema pallidum detection primer and kit

Abstract

The invention discloses a treponema pallidum detection primer and a kit. The characteristic that a treponema pallidum polA gene is highly conservative among different treponema pallidum subspecies isutilized; by optimally designing primers and probes of polA gene loci, the detection specificity of the treponema pallidum is guaranteed, the treponema pallidum is detected by combining the primers and probes with a digital PCR technology, the detection sensitivity and timeliness are greatly improved, and powerful help is provided for treatment effect monitoring and treatment prognosis judgment.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a treponema pallidum detection primer and a kit. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum infection of Treponemaceae and Treponema genus. The pathogen is also called Treponema pallidum because it is transparent in color under a microscope and is not easily stained by dyes. Syphilis was first discovered in North America and introduced to China in the 16th century. It once became the top four venereal diseases in China before liberation, and was basically eliminated in the middle of the 20th century. However, since the 1980s, syphilis has reappeared in China, and the number of reported cases of syphilis in the country has increased significantly again, and the prevalence has increased rapidly year by year. From 80,506 new cases in 1999 to 327,433 new cases in 2009, the incidence rate increased at an average rate of 14.3%...

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Problems solved by technology

However, due to the limitation of the detection principle of fluorescent quantitative PCR, the detection limit of this detection method is still difficult to reach a single copy, and the detection effect for the early stage of the disease is still lacking

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