Treponema pallidum detection primer and kit

A Treponema pallidum and detection kit technology, applied in the field of molecular biology, can solve the problems of lack of detection effect, the detection limit of detection method is difficult to reach a single copy, etc., shorten the detection window period, facilitate discovery, diagnosis and treatment, and ensure specificity sexual effect

Inactive Publication Date: 2020-04-03
SUZHOU TEST DROP DX BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the limitation of the detection principle of fluorescent quantitative PCR, the detection limit of this detection method is still difficult to reach a single copy, and the detection effect for the early stage of the disease is still lacking

Method used

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  • Treponema pallidum detection primer and kit
  • Treponema pallidum detection primer and kit
  • Treponema pallidum detection primer and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. The samples used in this embodiment include 2 cases of Treponema pallidum positive nucleic acid solution and 1 case of negative nucleic acid solution, which are respectively numbered as sample No. 1, sample No. 2 and sample No. 3.

[0048] 2. Preparation of PCR reaction system

[0049] The droplet generation device used in this example is specifically made of PC material, with a length of 30mm, a width of 25mm, and a thickness of 2mm. Well 3 has a volume of 100 μL. Its structure is as figure 1 As shown, other commercially available droplet generation products can also be used, which is not limited here.

[0050] (1) Take out the PCR reaction solution from the refrigerator in advance to thaw, vortex and mix well after melting completely, and centrifuge instantaneously;

[0051] (2) Take 20 μL of PCR reaction solution and place it in a PCR tube, add 5 μL of nucleic acid sample into it, and mix well with a pipette tip;

[0052] Table 3. Composition system of 20μL PC...

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Abstract

The invention discloses a treponema pallidum detection primer and a kit. The characteristic that a treponema pallidum polA gene is highly conservative among different treponema pallidum subspecies isutilized; by optimally designing primers and probes of polA gene loci, the detection specificity of the treponema pallidum is guaranteed, the treponema pallidum is detected by combining the primers and probes with a digital PCR technology, the detection sensitivity and timeliness are greatly improved, and powerful help is provided for treatment effect monitoring and treatment prognosis judgment.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a treponema pallidum detection primer and a kit. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum infection of Treponemaceae and Treponema genus. The pathogen is also called Treponema pallidum because it is transparent in color under a microscope and is not easily stained by dyes. Syphilis was first discovered in North America and introduced to China in the 16th century. It once became the top four venereal diseases in China before liberation, and was basically eliminated in the middle of the 20th century. However, since the 1980s, syphilis has reappeared in China, and the number of reported cases of syphilis in the country has increased significantly again, and the prevalence has increased rapidly year by year. From 80,506 new cases in 1999 to 327,433 new cases in 2009, the incidence rate increased at an average rate of 14.3%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/06C12N15/11
CPCC12Q1/689C12Q1/6851C12Q2600/166C12Q2563/159C12Q2563/107C12Q2545/113
Inventor 张惠丹戴敬节淳皓
Owner SUZHOU TEST DROP DX BIOTECHNOLOGY CO LTD
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